G0 Cell Unit, Okinawa Institute of Science and Technology Graduate University (OIST), Okinawa, Japan.
R&D Cluster Programs Section, Technology Development and Innovation Center, Okinawa Institute of Science and Technology Graduate University (OIST), Okinawa, Japan.
Sci Rep. 2023 Feb 11;13(1):2460. doi: 10.1038/s41598-023-29619-4.
Definitive differences in blood metabolite profiles between obese and non-obese Type 2 diabetes (T2D) have not been established. We performed an LC-MS-based non-targeted metabolomic analysis of whole blood samples collected from subjects classified into 4 types, based on the presence or absence of obesity and T2D. Of the 125 compounds identified, 20, comprising mainly nucleobases and glucose metabolites, showed significant increases or decreases in the T2D group. These included cytidine, UDP-glucuronate, UMP, 6-phosphogluconate, and pentose-phosphate. Among those 20 compounds, 11 enriched in red blood cells (RBCs) have rarely been studied in the context of diabetes, indicating that RBC metabolism is more extensively disrupted than previously known. Correlation analysis revealed that these T2D markers include 15 HbA1c-associated and 5 irrelevant compounds that may reflect diabetic conditions by a different mechanism than that of HbA1c. In the obese group, enhanced protein and fatty acid catabolism causes increases in 13 compounds, including methylated or acetylated amino acids and short-chain carnitines. Our study, which may be considered a pilot investigation, suggests that changes in blood metabolism due to obesity and diabetes are large, but essentially independent.
肥胖和非肥胖 2 型糖尿病(T2D)患者的血液代谢物谱存在明确差异尚未确定。我们根据是否存在肥胖和 T2D 将受试者分为 4 种类型,对采集的全血样本进行了基于 LC-MS 的非靶向代谢组学分析。在所鉴定的 125 种化合物中,20 种主要由核苷碱基和葡萄糖代谢物组成的化合物在 T2D 组中显示出显著增加或减少。其中包括胞苷、UDP-葡萄糖醛酸、UMP、6-磷酸葡萄糖酸和戊糖磷酸。在这 20 种化合物中,有 11 种在 RBC 中富集,在糖尿病背景下很少被研究,这表明 RBC 代谢的紊乱程度比以前所知的更为广泛。相关性分析表明,这些 T2D 标志物包括 15 种与 HbA1c 相关的和 5 种不相关的化合物,它们可能通过与 HbA1c 不同的机制反映糖尿病状态。在肥胖组中,增强的蛋白质和脂肪酸分解代谢导致 13 种化合物的增加,包括甲基化或乙酰化氨基酸和短链肉碱。我们的研究可以被认为是一项初步研究,表明肥胖和糖尿病引起的血液代谢变化很大,但基本上是独立的。