Pitol-Palin Letícia, de Souza Batista Fábio Roberto, Gomes-Ferreira Pedro Henrique Silva, Mulinari-Santos Gabriel, Ervolino Edilson, Souza Francisley Ávila, Matsushita Dóris Hissako, Okamoto Roberta
Department of Diagnosis and Surgery, Araçatuba Dental School, São Paulo State University Júlio de Mesquita Filho, Araçatuba 16015-050, Brazil.
Department of Basic Sciences, Araçatuba Dental School, São Paulo State University Júlio de Mesquita Filho, Araçatuba 16066-840, Brazil.
Biology (Basel). 2020 Dec 16;9(12):471. doi: 10.3390/biology9120471.
The aim of this study was to analyze the stages of the alveolar bone repair in type 2 diabetic rats evaluating the mechanism of mineralization and bone remodeling processes after dental extraction. Forty-eight rats were divided into normoglycemic (NG) and type 2 diabetes (T2D) groups. The upper right incisor was extracted and after 3, 7, 14 and 42 days the animals were euthanized. The following analyses were performed: immunolabeling against antibodies TNFα, TGFβ, IL6, WNT, OCN and TRAP, collagen fibers maturation, microtomography and confocal microscopy. Data were submitted to statistical analysis. The immunolabeling analysis showed that the T2D presented a more pronounced alveolar inflammation than NG. Labeling of proteins responsible for bone formation and mineralization was higher in NG than T2D, which presented greater resorptive activity characterized by TRAP labeling. Also, T2D group showed a decrease in the amount of collagen fibers. Micro-CT analysis showed that T2D causes a decrease in bone volume percentage due to deficient trabecular parameters and higher porosity. The T2D bone dynamics show a loss in bone remodeling process. T2D prolongs the local inflammatory process, which impairs the organization and maturation of collagen fibers, delaying bone formation that generates impact on mineralization and bone turnover.
本研究的目的是分析2型糖尿病大鼠牙槽骨修复的阶段，评估拔牙后矿化和骨重塑过程的机制。48只大鼠被分为正常血糖（NG）组和2型糖尿病（T2D）组。拔除右上切牙，在3、7、14和42天后对动物实施安乐死。进行了以下分析：针对肿瘤坏死因子α（TNFα）、转化生长因子β（TGFβ）、白细胞介素6（IL6）、WNT、骨钙素（OCN）和抗酒石酸酸性磷酸酶（TRAP）抗体的免疫标记、胶原纤维成熟度、显微断层扫描和共聚焦显微镜检查。数据进行了统计分析。免疫标记分析表明，T2D组的牙槽炎症比NG组更明显。负责骨形成和矿化的蛋白质标记在NG组高于T2D组，T2D组以TRAP标记为特征表现出更强的吸收活性。此外，T2D组胶原纤维数量减少。显微CT分析表明，由于小梁参数不足和孔隙率较高，T2D导致骨体积百分比降低。T2D组的骨动态显示骨重塑过程受损。T2D延长了局部炎症过程，这损害了胶原纤维的组织和成熟，延迟了骨形成，对矿化和骨转换产生影响。
