Kim Sung-Hyun, Lee Dong Han, Lee Jin Hee, Yang Jun-Young, Shin Hyo-Sook, Lee JeongPyo, Jung Kikyung, Jeong Jayoung, Oh Jae-Ho, Lee Jong Kwon
Division of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju 28159, Chungcheongbuk-do, Korea.
Toxics. 2020 Dec 16;8(4):122. doi: 10.3390/toxics8040122.
Carbon nanotubes (CNTs) are one of the major types of nanomaterials that have various industrial and biomedical applications. However, there is a risk of accidental exposure to CNTs in individuals involved in their large-scale production and in individuals who use products containing CNTs. This study aimed to evaluate the skin sensitization induced by CNTs using two alternative tests. We selected single-wall carbon nanotubes and multi-walled carbon nanotubes for this study. First, the physiochemical properties of the CNTs were measured, including the morphology, size, and zeta potential, under various conditions. Thereafter, we assessed the sensitization potential of the CNTs using the ARE-Nrf2 Luciferase KeratinoSens™ assay, an in vitro alternative test method. In addition, the CNTs were evaluated for their skin sensitization potential using the LLNA: BrdU-FCM in vivo alternative test method. In this study, we report for the first time the sensitization results of CNTs using the KeratinoSens™ and LLNA: BrdU-FCM test methods in this study. This study found that both CNTs do not induce skin sensitization. These results suggest that the KeratinoSens™ and LLNA: BrdU-FCM assay may be useful as alternative assays for evaluating the potential of some nanomaterials that can induce skin sensitization.
碳纳米管（CNTs）是具有多种工业和生物医学应用的主要纳米材料类型之一。然而，参与其大规模生产的人员以及使用含碳纳米管产品的人员存在意外接触碳纳米管的风险。本研究旨在使用两种替代试验评估碳纳米管引起的皮肤致敏作用。我们选择了单壁碳纳米管和多壁碳纳米管进行本研究。首先，在各种条件下测量了碳纳米管的物理化学性质，包括形态、尺寸和zeta电位。此后，我们使用ARE-Nrf2荧光素酶角质形成细胞传感（KeratinoSens™）试验（一种体外替代试验方法）评估了碳纳米管的致敏潜力。此外，使用LLNA:BrdU-FCM体内替代试验方法评估了碳纳米管的皮肤致敏潜力。在本研究中，我们首次报告了使用KeratinoSens™和LLNA:BrdU-FCM试验方法对碳纳米管进行致敏试验的结果。本研究发现，两种碳纳米管均不会引起皮肤致敏。这些结果表明，KeratinoSens™和LLNA:BrdU-FCM试验可作为评估某些可能引起皮肤致敏的纳米材料潜力的替代试验。
