Marra Roberta, Coppola Mariangela, Pironti Angela, Grasso Filomena, Lombardi Nadia, d'Errico Giada, Sicari Andrea, Bolletti Censi Sergio, Woo Sheridan L, Rao Rosa, Vinale Francesco
Department of Agricultural Sciences, University of Naples Federico II, Portici, 80055 Naples, Italy.
BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Naples Federico II, Portici, 80055 Naples, Italy.
J Fungi (Basel). 2020 Dec 16;6(4):369. doi: 10.3390/jof6040369.
Biocontrol fungal strains of the genus can antagonize numerous plant pathogens and promote plant growth using different mechanisms of action, including the production of secondary metabolites (SMs). In this work we analyzed the effects of repeated applications of selected strains or SMs on young olive trees on the stimulation of plant growth and on the development of olive leaf spot disease caused by . In addition, metabolomic analyses and gene expression profiles of olive leaves were carried out by LC-MS Q-TOF and real-time RT-PCR, respectively. A total of 104 phenolic compounds were detected from olive leave extracts and 20 were putatively identified. Targeted and untargeted approaches revealed significant differences in both the number and type of phenolic compounds accumulated in olive leaves after applications, as compared to water-treated plants. Different secoiridoids were less abundant in treated plants than in controls, while the accumulation of flavonoids (including luteolin and apigenin derivatives) increased following the application of specific strain. The induction of defense-related genes, and of genes involved in the synthesis of the secoiridoid oleuropein, was also analyzed and revealed a significant variation of gene expression according to the strain or metabolite applied.
该属的生物防治真菌菌株可以通过不同的作用机制拮抗多种植物病原体并促进植物生长，这些机制包括次生代谢产物（SMs）的产生。在这项工作中，我们分析了对幼龄橄榄树重复施用选定的菌株或次生代谢产物对植物生长刺激以及由引起的橄榄叶斑病发展的影响。此外，分别通过液相色谱-质谱联用飞行时间质谱仪（LC-MS Q-TOF）和实时逆转录聚合酶链反应（real-time RT-PCR）对橄榄叶进行了代谢组学分析和基因表达谱分析。从橄榄叶提取物中检测到总共104种酚类化合物，其中20种被初步鉴定。靶向和非靶向方法显示，与水处理植物相比，施用后橄榄叶中积累的酚类化合物在数量和类型上均存在显著差异。处理过的植物中不同的裂环烯醚萜类化合物比对照植物中的含量少，而在施用特定菌株后，黄酮类化合物（包括木犀草素和芹菜素衍生物）的积累增加。还分析了防御相关基因以及参与裂环烯醚萜类橄榄苦苷合成的基因的诱导情况，结果表明基因表达根据所施用的菌株或代谢产物而有显著变化。
