染色质亚基的结构：粘质沙雷氏菌核酸内切酶研究

Pospelov V A, Svetlikova S B, Vorob'ev V I

Nucleic Acids Res. 1977 Sep;4(9):3267-79. doi: 10.1093/nar/4.9.3267.

Electrophoretic properties of chromatin subunits--nucleosomes--obtained by treatment of chromatin with the Serratia marcescens endonuclease have been studied. Double-stranded breaks of DNA between adjacent nucleosomes do not necessarily lead to their disjunction. Fragmentation of the DNA within the nucleosomes may proceed simultaneously with the breakdown of the DNA between the nucleosomes at early stages of the endonuclease digestion. Electrophoretic mobility and chromatographic properties of mononucleosomes, their dimers and trimers with internally degraded DNA was not changed. It has been deduced that the integrity of chromatin particles with internally fragmented DNA is supported by histone interaction inside and between the nucleosomes.

对通过粘质沙雷氏菌核酸内切酶处理染色质得到的染色质亚基——核小体的电泳性质进行了研究。相邻核小体之间DNA的双链断裂不一定会导致它们分离。在核酸内切酶消化的早期阶段，核小体内DNA的片段化可能与核小体之间DNA的断裂同时进行。单核小体、其含有内部降解DNA的二聚体和三聚体的电泳迁移率和色谱性质没有改变。据推断，具有内部片段化DNA的染色质颗粒的完整性是由核小体内和核小体之间的组蛋白相互作用维持的。