Unidad de Investigación Multidisciplinaria L14 (Alimentos, Micotoxinas, y Micotoxicosis), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, State of Mexico 54714, Mexico.
Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
Food Chem. 2021 May 30;345:128863. doi: 10.1016/j.foodchem.2020.128863. Epub 2020 Dec 13.
In vitro experiments were conducted to evaluate the effectiveness of two new biosorbents (lettuce and field horsetail) in removing aflatoxin B (AFB). Formosa firethorn was used as reference material. The adsorption of AFB (190 ng/mL) was investigated at two sorbent contents (0.5% and 0.1% w/v) and three pHs (2, 5, and 7). Batch experiments were performed at 40 °C for 2 h. Several methodologies were used to characterize the nature of the biosorbent-AFB interaction. In general, when using biosorbents at 0.5% w/v, AFB was well adsorbed by the three tested biomaterials (70 to 100%). Furthermore, with the lowest biosorbent content (0.1% w/v), significant AFB adsorption efficiencies were attained at pH 5 (33 to 50%). Nevertheless, at pH 7, lettuce showed the highest ability against AFB removal (95%). Further characterization of the AFB-loaded biosorbents demonstrated that chemical and physical mechanisms were involved in the adsorption process.
进行了体外实验以评估两种新型生物吸附剂（生菜和木贼）去除黄曲霉毒素 B（AFB）的效果。以 Formosa firethorn 作为参考材料。在两种吸附剂含量（0.5%和 0.1% w/v）和三种 pH 值（2、5 和 7）下研究了 AFB（190 ng/mL）的吸附情况。在 40°C 下进行了 2 小时的分批实验。采用了几种方法来表征生物吸附剂-AFB 相互作用的性质。一般来说，当使用 0.5% w/v 的生物吸附剂时，三种测试生物材料对 AFB 的吸附效果良好（70%至 100%）。此外，在最低生物吸附剂含量（0.1% w/v）下，在 pH 5 时可达到显著的 AFB 吸附效率（33%至 50%）。然而，在 pH 7 时，生菜对 AFB 的去除能力最高（95%）。对负载 AFB 的生物吸附剂的进一步表征表明，化学和物理机制都参与了吸附过程。
