Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Real de Juriquilla, Queretaro 76230, Mexico.
National Autonomous University of Mexico-Superior Studies Faculty at Cuautitlan (UNAM⁻FESC), Multidisciplinary Research Unit L5, Cuautitlan Izcalli 54714, Mexico.
Toxins (Basel). 2018 Nov 21;10(11):484. doi: 10.3390/toxins10110484.
Experiments were carried out to evaluate the effectiveness of three different biosorbents (banana peel, leaves, and powder) in removing aflatoxin B₁ (AFB₁). A noncommercial mycotoxin binder (zeolite) was used as a reference material. A laboratory model that simulated the in vivo conditions of the poultry gastrointestinal tract was utilized to prove the removal efficiency of the biosorbents when added to AFB₁-contaminated diet (100 µg/kg). The concentration of AFB₁ was determined using antibody-based immunoaffinity column and spectrofluorometry methodologies. Z potential (ζ), point of zero charge (pH), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy with attenuated total reflection (FTIR-ATR), and UV-Vis diffuse reflectance spectroscopy (DRS) techniques were used to further characterize the biosorbents. The addition of the biosorbents (1.5%, /) to the diet significantly reduced the bioavailability of AFB₁ in the intestinal section. The highest aflatoxin adsorption values were 69% and 70% using powder and zeolite, respectively. A moderate biosorption uptake of 46% was achieved using leaves. The biomaterial with the lowest removal capacity was banana peel (28%). In conclusion, powder could be used as an alternative to conventional systems for AFB₁ removal.
进行了实验以评估三种不同生物吸附剂（香蕉皮、叶和粉）在去除黄曲霉毒素 B₁（AFB₁）方面的效果。使用非商业的霉菌毒素结合剂（沸石）作为参考材料。利用模拟家禽胃肠道体内条件的实验室模型，证明了生物吸附剂在添加到受 AFB₁污染的饮食（100 µg/kg）中的去除效率。使用基于抗体的免疫亲和柱和荧光光谱法测定 AFB₁ 的浓度。采用 Zeta 电位（ζ）、零电荷点（pH）、能量色散 X 射线光谱（EDS）、X 射线衍射（XRD）、衰减全反射傅里叶变换红外光谱（FTIR-ATR）和紫外可见漫反射光谱（DRS）技术进一步对生物吸附剂进行了表征。将生物吸附剂（1.5%，/）添加到饮食中，可显著降低肠道段中 AFB₁ 的生物利用度。使用 粉和沸石，黄曲霉毒素吸附值最高分别为 69%和 70%。叶的生物吸附吸收率适中，为 46%。去除能力最低的生物材料是香蕉皮（28%）。总之，粉可作为去除 AFB₁ 的常规系统的替代品。
