石榴果皮凝集素（PgTeL）对单核细胞增生李斯特菌的抑菌作用。
Antibacterial effects of the lectin from pomegranate sarcotesta (PgTeL) against Listeria monocytogenes.
机构信息
Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil.
Departamento de Biofísica e Radiobiologia, Centro de Biociências, Universidade Federal de Pernambuco, Recife, Brazil.
出版信息
J Appl Microbiol. 2021 Aug;131(2):671-681. doi: 10.1111/jam.14978. Epub 2021 Jan 5.
AIMS
To investigate the effects of the lectin from Punica granatum sarcotesta (PgTeL) on growth, viability, cell structure, biofilm formation and chitinase activity of Listeria monocytogenes. In addition, the effect of PgTeL on the adhesion and invasion of human cells (HeLa) was determined.
METHODS AND RESULTS
PgTeL showed bacteriostatic and bactericidal effects on the strains L. monocytogenes N53-1 and EGD-e, causing morphometric alterations, cell aggregation, strong deformation and cell disruption. PgTeL inhibited biofilm formation by EGD-e and N53-1 and also interfered with the adhesion and invasion processes of EGD-e and N53-1 in HeLa cells. Finally, the chitinase activity of L. monocytogenes EGD-e was reduced in the presence of PgTeL, which can be involved in the inhibition of adhesion process.
CONCLUSION
PgTeL is an antibacterial agent against L. monocytogenes, inhibiting growth and promoting cell death, as well as impairing biofilm formation and bacterial adhesion and invasion into human cells.
SIGNIFICANCE AND IMPACT OF THE STUDY
The results stimulate future investigations on the potential of PgTeL for protection of contamination in food products.
目的
研究石榴皮凝集素（PgTeL）对单核细胞增生李斯特菌生长、活力、细胞结构、生物膜形成和几丁质酶活性的影响。此外，还测定了 PgTeL 对人细胞（HeLa）黏附和侵袭的影响。
方法与结果
PgTeL 对单核细胞增生李斯特菌 N53-1 和 EGD-e 菌株表现出抑菌和杀菌作用，导致形态计量学改变、细胞聚集、强烈变形和细胞破裂。PgTeL 抑制了 EGD-e 和 N53-1 的生物膜形成，并且还干扰了 EGD-e 和 N53-1 在 HeLa 细胞中的黏附和侵袭过程。最后，PgTeL 降低了单核细胞增生李斯特菌 EGD-e 的几丁质酶活性，这可能参与了对黏附过程的抑制。
结论
PgTeL 是一种抗单核细胞增生李斯特菌的抗菌剂，可抑制生长并促进细胞死亡，同时破坏生物膜形成以及细菌对人细胞的黏附和侵袭。
研究的意义和影响
研究结果激发了对 PgTeL 用于保护食品产品污染的潜在应用的进一步研究。
Zotero 插件