Centre of Excellence in Molecular Biology and Regenerative Medicine (DST-FIST Supported Centre), Department of Biochemistry (DST-FIST Supported Department), JSS Medical College, JSS Academy of Higher Education and Research (JSS AHER); Department of Molecular Biology, Yuvaraja's College, University of Mysore, Mysuru, Karnataka, India.
Centre of Excellence in Molecular Biology and Regenerative Medicine (DST-FIST Supported Centre), Department of Biochemistry (DST-FIST Supported Department), JSS Medical College, JSS Academy of Higher Education and Research (JSS AHER), Mysuru, Karnataka, India.
J Cancer Res Ther. 2020 Oct-Dec;16(6):1235-1249. doi: 10.4103/jcrt.JCRT_494_19.
Annona muricata (L.) (AM), commonly known as Soursop and Lakshmanaphala/Hanumaphala in India, has been extensively used in ethnomedicine for treating tuberculosis, urinary tract infections (UTIs) and cancers. The fruit is a rich source of antioxidants and antitumor agents.
In this study, we have extracted phytochemicals that exhibited anti-cancer property from the (a) fruit pulp using methanol (AMPM) and water (AMPW); and (b) seeds using methanol (AMSM). Qualitative phytochemical analysis showed the presence of phenolics, tannins, alkaloids, flavonoids, sterols, terpenoids, carbohydrates and proteins in AMPM and AMPW. All three extracts were first checked for in vitro antioxidant and anti-inflammatory properties and then tested for efficacy against MCF-7 and MDA-MB-231.
Among these three extracts, AMSM showed the highest antioxidant power as well as ~80% inhibition at 320μg/ml concentration in both cell lines upon treatment for 24h. However, only about 40% inhibition was observed with 320μg/ml AMPM treatment, despite its highest anti-inflammatory potential. Water extract AMPW exhibited about 80% growth inhibition at 50% dilution. Since fruit pulp is the one consumed, the extracts AMPM and AMPW were further tested for apoptosis induction and cell cycle arrest. Analysis of the data showed increased apoptosis and G0/G1 cell cycle arrest upon exposure to AMPM and AMPW.
安农西亚（Annona muricata）（AM），俗称刺果番荔枝，在印度被广泛应用于民间医学，用于治疗肺结核、尿路感染（UTIs）和癌症。这种水果富含抗氧化剂和抗肿瘤剂。
在这项研究中，我们从（a）果肉中用甲醇（AMPM）和水（AMPW）；以及（b）种子中用甲醇（AMSM）提取具有抗癌特性的植物化学物质。定性植物化学分析表明，AMPM 和 AMPW 中存在酚类、单宁、生物碱、类黄酮、甾醇、萜类、碳水化合物和蛋白质。首先检查这三种提取物的体外抗氧化和抗炎特性，然后测试它们对 MCF-7 和 MDA-MB-231 的疗效。
在这三种提取物中，AMSM 在两种细胞系中表现出最高的抗氧化能力，在 320μg/ml 浓度下处理 24 小时后，其抑制率约为 80%。然而，尽管 AMPM 具有最高的抗炎潜力，但在 320μg/ml 的 AMPM 处理下，仅观察到约 40%的抑制。水提取物 AMPW 在 50%稀释时表现出约 80%的生长抑制。由于果肉是被消耗的，因此进一步测试了 AMPM 和 AMPW 对细胞凋亡和细胞周期阻滞的诱导作用。数据分析表明，暴露于 AMPM 和 AMPW 后，细胞凋亡增加，G0/G1 细胞周期阻滞。
