Institute of Nuclear Physics Polish Academy of Sciences, 31-342 Krakow, Poland.
Department of Clinical Immunology, Jagiellonian University Medical College, 30-663 Krakow, Poland.
Int J Mol Sci. 2020 Dec 17;21(24):9610. doi: 10.3390/ijms21249610.
Enhancing the effectiveness of colorectal cancer treatment is highly desirable. Radiation-based anticancer therapy-such as proton therapy (PT)-can be used to shrink tumors before subsequent surgical intervention; therefore, improving the effectiveness of this treatment is crucial. The addition of noble metal nanoparticles (NPs), acting as radiosensitizers, increases the PT therapeutic effect. Thus, in this paper, the effect of novel, gold-platinum nanocauliflowers (AuPt NCs) on PT efficiency is determined. For this purpose, crystalline, 66-nm fancy shaped, bimetallic AuPt NCs were synthesized using green chemistry method. Then, physicochemical characterization of the obtained AuPt NCs by transmission electron microscopy (TEM), selected area electron diffraction (SAED), energy dispersive X-ray spectroscopy (EDS), and UV-Vis spectra measurements was carried out. Fully characterized AuPt NCs were placed into a cell culture of colon cancer cell lines (HCT116, SW480, and SW620) and a normal colon cell line (FHC) and subsequently subjected to proton irradiation with a total dose of 15 Gy. The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) test, performed after 18-h incubation of the irradiated cell culture with AuPt NCs, showed a significant reduction in cancer cell viability compared to normal cells. Thus, the radio-enhancing features of AuPt NCs indicate their potential application for the improvement in effectiveness of anticancer proton therapy.
提高结直肠癌治疗效果是非常可取的。基于辐射的抗癌疗法，如质子治疗（PT），可以在随后的手术干预前缩小肿瘤；因此，提高这种治疗的效果至关重要。添加作为放射增敏剂的贵金属纳米颗粒（NPs）可以提高 PT 的治疗效果。因此，在本文中，确定了新型金铂纳米冠（AuPt NCs）对 PT 效率的影响。为此，使用绿色化学方法合成了结晶的、66nm 奇特形状的双金属 AuPt NCs。然后，通过透射电子显微镜（TEM）、选区电子衍射（SAED）、能谱（EDS）和紫外可见光谱测量对所获得的 AuPt NCs 的物理化学特性进行了表征。对完全表征的 AuPt NCs 进行细胞培养，包括结肠癌细胞系（HCT116、SW480 和 SW620）和正常结肠细胞系（FHC），然后用总剂量为 15 Gy 的质子进行辐照。将 AuPt NCs 与辐照后的细胞培养物孵育 18 小时后进行 3-（4,5-二甲基噻唑-2-基）-5-（3-羧甲基苯氧基）-2-（4-磺基苯）-2H-四唑（MTS）试验，结果表明与正常细胞相比，癌细胞活力显著降低。因此，AuPt NCs 的放射增强特性表明它们具有潜在的应用价值，可以提高抗癌质子治疗的效果。
