Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russian Federation.
Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russian Federation.
Carbohydr Polym. 2020 Apr 15;234:115916. doi: 10.1016/j.carbpol.2020.115916. Epub 2020 Jan 25.
In presented study, various chitosan derivatives containing covalently bounded gallic acid were obtained: chitosan with gallic acid (CG), quaternized chitosan with gallic acid (QCG), and succinylated chitosan with gallic acid (SCG). Chitosan derivatives were used as stabilizing and reducing agents in the synthesis of silver nanoparticles (AgNPs). The dimensional characteristics of nanomaterials were determined by transmission electron (TEM), dynamic light scattering (DLS) and atomic force (AFM) microscopy, antibacterial activity (against E. coli, S. epidermidis), cytotoxicity (HaCaT, Colo 357 cell lines) and hemocompatibility. Among all samples, QCG-AgNPs showed low toxicity in the range of studied concentrations (3.125-100 μg/ml) high stability of nanoparticle for 4 months (according to UV.spectroscopy data) the highest antibacterial activity against S. epidermidis (3.91 μg/ml). The high antibacterial activity, stability, and simplicity of the process of producing AgNPs based on the QCG derivative reveals that a new method for producing modified AgNPs deserves future consideration.
在本研究中，获得了各种含有共价结合没食子酸的壳聚糖衍生物：壳聚糖与没食子酸（CG）、壳聚糖与没食子酸季铵盐（QCG）和壳聚糖与没食子酸琥珀酸酯（SCG）。壳聚糖衍生物被用作银纳米粒子（AgNPs）合成中的稳定剂和还原剂。纳米材料的尺寸特征通过透射电子显微镜（TEM）、动态光散射（DLS）和原子力显微镜（AFM）进行测定，同时还评估了它们的抗菌活性（针对大肠杆菌、表皮葡萄球菌）、细胞毒性（HaCaT、Colo 357 细胞系）和血液相容性。在所有样品中，QCG-AgNPs 在研究浓度范围内（3.125-100μg/ml）表现出低毒性，纳米粒子的稳定性高，可维持 4 个月（根据 UV 光谱数据），对表皮葡萄球菌的抗菌活性最高（3.91μg/ml）。基于 QCG 衍生物生产 AgNPs 的方法具有高效抗菌活性、稳定性和简单性，这表明这种新方法值得进一步考虑用于生产修饰后的 AgNPs。
