Mendez-Pfeiffer Pablo, Alday Efrain, Carreño Ana Laura, Hernández-Tánori Jorge, Montaño-Leyva Beatriz, Ortega-García Jesús, Valdez Judith, Garibay-Escobar Adriana, Hernandez Javier, Valencia Dora, Velazquez Carlos
Department of Chemistry-Biology, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Hermosillo, Sonora C.P. 83000, Mexico.
Department of Chemical Biological and Agropecuary Sciences, University of Sonora, Av. Universidad and Irigoyen, Caborca, Sonora C.P. 83600, Mexico.
Antioxidants (Basel). 2020 Dec 17;9(12):1294. doi: 10.3390/antiox9121294.
The main chemical composition and pharmacological potential of propolis from arid and semi-arid regions of the Sonoran Desert have been previously reported. Caborca propolis (CP), from an arid zone of the Sonoran Desert, has shown a polyphenolic profile that suggests a mixed plant origin, presenting poplar-type markers, as well as a 6-methoxylated flavonoid, xanthomicrol, characteristic of Asteraceae plants. In addition, CP has shown significant antioxidant properties and antiproliferative activity on cancer cells. In this study, we analyzed the influence of collection time on the chemical constitution, antiproliferative activity and protective capacity of CP against reactive oxygen species (ROS), by using HPLC-UV-diode array detection (DAD) analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-Dimethyltetrazoliumbromide (MTT) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays, as well as cellular antioxidant activity (CAA) assay on murine B-cell lymphoma M12.C3.F6 cells. HPLC-UV-DAD analyses of seasonally collected CP (one-year period) revealed quantitative differences among the most abundant CP constituents: pinocembrin, galangin, chrysin and pinobanksin-3--acetate. Though all seasonal samples of CP induced an antiproliferative effect in M12.C3.F6 cells, CP from autumn showed the highest inhibitory activity (IC: 5.9 ± 0.6 µg/mL). The DPPH assay pointed out that CP collected in autumn presented the highest antioxidant potential (IC: 58.8 ± 6.7 µg/mL), followed by winter (65.7 ± 12.2 µg/mL) and spring (67.0 ± 7.5 µg/mL); meanwhile, the summer sample showed a lesser antioxidant capacity (IC: 98.7 ± 2.5 µg/mL). The CAA assay demonstrated that CP induced a significant protective effect against ROS production elicited by HO in M12.C3.F6 cells. Pretreatment of M12.C3.F6 cells with CP from spring and autumn (25 and 50 µg/mL for 1 h) showed the highest reduction in intracellular ROS induced by HO (1 and 5 mM). These results indicate that the antiproliferative effect and cellular antioxidant activity of CP are modulated by quantitative fluctuations in its polyphenolic profile due to its collection time.
先前已有关于索诺兰沙漠干旱和半干旱地区蜂胶的主要化学成分及药理潜力的报道。来自索诺兰沙漠干旱地区的卡波卡蜂胶（CP）呈现出一种多酚谱，表明其植物来源混合，含有杨树型标志物以及菊科植物特有的6-甲氧基黄酮黄腐醇。此外，CP已显示出显著的抗氧化特性和对癌细胞的抗增殖活性。在本研究中，我们通过高效液相色谱-紫外-二极管阵列检测（DAD）分析、3-(4,5-二甲基噻唑-2-基)-2,5-二甲基四氮唑溴盐（MTT）和2,2-二苯基-1-苦基肼（DPPH）测定，以及对小鼠B细胞淋巴瘤M12.C3.F6细胞的细胞抗氧化活性（CAA）测定，分析了采集时间对CP的化学组成、抗增殖活性及对活性氧（ROS）的保护能力的影响。对季节性采集的CP（为期一年）进行的高效液相色谱-紫外-DAD分析揭示了CP中最丰富成分（松属素、高良姜素、白杨素和松属素-3-乙酸酯）之间的定量差异。尽管CP的所有季节性样品均在M12.C3.F6细胞中诱导了抗增殖作用，但秋季的CP显示出最高的抑制活性（IC：5.9±0.6μg/mL）。DPPH测定指出，秋季采集的CP具有最高的抗氧化潜力（IC：58.8±6.7μg/mL），其次是冬季（65.7±12.2μg/mL）和春季（67.0±7.5μg/mL）；同时，夏季样品的抗氧化能力较低（IC：98.7±2.5μg/mL）。CAA测定表明，CP对M12.C3.F6细胞中由HO引发的ROS产生具有显著的保护作用。用春季和秋季的CP（25和50μg/mL，处理1小时）预处理M12.C3.F6细胞，显示出对由HO（1和5mM）诱导的细胞内ROS的最大降低。这些结果表明，CP的抗增殖作用和细胞抗氧化活性因其采集时间导致的多酚谱定量波动而受到调节。
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