Danielsen H E, Steen H B, Lindmo T, Reith A
Department of Pathology, Norwegian Radium Hospital, Montebello, Oslo.
Carcinogenesis. 1988 Jan;9(1):59-63. doi: 10.1093/carcin/9.1.59.
There have been several reports on the distribution of DNA in premalignant and malignant liver cells after carcinogen treatment in rodents. The most striking result so far is that ploidy distributions seems to depend more on experimental protocols and less on carcinogenesis per se. The present study demonstrates that an evaluation by means of flow cytometry and stereological image analysis yields results showing similar ploidy distributions throughout the known sequence of tissue changes seen during liver cancer development. Furthermore, our results demonstrate inter-individual differences between the lesions in each of the steps in these tissue changes, (foci, nodules and hepatocarcinomas). The present study gives no reason to conclude that changes in ploidy distribution are essential in liver carcinogenesis.
关于啮齿动物经致癌物处理后癌前和恶性肝细胞中DNA分布的报道已有多篇。目前最显著的结果是，倍性分布似乎更多地取决于实验方案，而较少取决于致癌作用本身。本研究表明，通过流式细胞术和体视学图像分析进行评估，所得结果显示，在肝癌发展过程中已知的组织变化序列中，倍性分布相似。此外，我们的结果表明，在这些组织变化的每个步骤（灶、结节和肝癌）中的病变之间存在个体差异。本研究没有理由得出倍性分布变化在肝癌发生中至关重要的结论。
