Chetkowski R J, Nass T E
Alta Bates Hospital, Berkeley, California 94705.
Fertil Steril. 1988 Jan;49(1):171-3.
In vitro development of 2-cell mouse embryos to the blastocyst stage was used to assess the toxicity of isofluorane, nitrous oxide, fentanyl, and meperidine. Isofluorane at concentrations similar to those employed during human oocyte recovery for IVF significantly inhibited mouse embryo development. Since the other agents were without effect, balanced anesthesia with nitrous oxide and narcotics may be preferable to isofluorane for IVF and GIFT.
利用二细胞期小鼠胚胎在体外发育至囊胚阶段来评估异氟烷、氧化亚氮、芬太尼和哌替啶的毒性。与人类体外受精（IVF）取卵时所使用浓度相似的异氟烷显著抑制了小鼠胚胎的发育。由于其他药物没有影响，对于IVF和配子输卵管内移植（GIFT）而言，采用氧化亚氮和麻醉性镇痛药的平衡麻醉可能比异氟烷更可取。
