Guambo María Paula Romero, Spencer Lilian, Vispo Nelson Santiago, Vizuete Karla, Debut Alexis, Whitehead Daniel C, Santos-Oliveira Ralph, Alexis Frank
School of Biological Sciences and Engineering, Yachay Tech University, Urcuquí, Imbabura 100115, Ecuador.
Center of Nanoscience and Nanotechnology, Universidad de las Fuerzas Armadas ESPE, Sangolquí 1715231, Ecuador.
Polymers (Basel). 2020 Dec 18;12(12):3042. doi: 10.3390/polym12123042.
Suture biomaterials are critical in wound repair by providing support to the healing of different tissues including vascular surgery, hemostasis, and plastic surgery. Important properties of a suture material include physical properties, handling characteristics, and biological response for successful performance. However, bacteria can bind to sutures and become a source of infection. For this reason, there is a need for new biomaterials for suture with antifouling properties. Here we report two types of cellulose fibers from coconut () and sisal (), which were purified with a chemical method, characterized, and tested in vitro and in vivo. According to SEM images, the cellulose fiber from coconut has a porous surface, and sisal has a uniform structure without internal spaces. It was found that the cellulose fiber from sisal has mechanical properties closer to silk fiber biomaterial using Ultimate Tensile Strength. When evaluating the cellulose fibers biodegradability, the cellulose from coconut showed a rapid weight loss compared to sisal. The antifouling test was negative, which demonstrated that neither possesses intrinsic microbicidal activity. Yet, a weak biofilm was formed on sisal cellulose fibers suggesting it possesses antifouling properties compared to cellulose from coconut. In vivo experiments using healthy mice demonstrated that the scarring and mechanical connection was like silk for both cellulose fibers. Overall, our results showed the potential use of cellulose fibers from vegetal for surgical sutures due to excellent mechanical properties, rapid degradation, and no bacterial adhesion.
缝合生物材料在伤口修复中至关重要，可为包括血管外科、止血和整形外科在内的不同组织的愈合提供支持。缝合材料的重要特性包括物理性能、操作特性以及为实现成功性能所需的生物学反应。然而，细菌可黏附于缝合线并成为感染源。因此，需要新型具有防污性能的缝合生物材料。在此，我们报告了两种来自椰子和剑麻的纤维素纤维，它们经过化学方法纯化、表征，并进行了体外和体内测试。根据扫描电子显微镜图像，椰子纤维素纤维表面多孔，剑麻纤维素纤维结构均匀且无内部空隙。研究发现，使用极限抗拉强度时，剑麻纤维素纤维的机械性能更接近丝纤维生物材料。在评估纤维素纤维的生物降解性时，与剑麻相比，椰子纤维素显示出更快的重量损失。防污测试呈阴性，这表明两者均不具有内在的杀菌活性。然而，剑麻纤维素纤维上形成了一层较弱的生物膜，这表明与椰子纤维素相比，它具有防污性能。使用健康小鼠进行的体内实验表明，两种纤维素纤维造成的瘢痕形成和机械连接情况均与丝线相似。总体而言，我们的结果表明，由于具有优异的机械性能、快速降解以及无细菌黏附特性，植物来源的纤维素纤维在外科缝合中有潜在应用价值。
