Hansson Oskar, Rutz Sandra, Zetterberg Henrik, Bauer Ekaterina, Hähl Teresa, Manuilova Ekaterina, Mert Mehmet Can, Wahl Simone, Blennow Kaj, Stomrud Erik
Clinical Memory Research Unit Department of Clinical Sciences Malmö Lund University Malmö Sweden.
Memory Clinic Skåne University Hospital Malmö Sweden.
Alzheimers Dement (Amst). 2020 Dec 18;12(1):e12137. doi: 10.1002/dad2.12137. eCollection 2020.
We aimed to establish a standardized, routine-use pre-analytical protocol for measuring Alzheimer's disease (AD) biomarkers in cerebrospinal fluid (CSF).
The effect of pre-analytical factors (sample collection/handling/storage/transportation) on biomarker levels was assessed using freshly collected CSF. Tube type/sterilization was assessed using previously frozen samples. A low-bind false-bottom tube (FBT, Sarstedt) was used for all experiments, except tube types/sterilization experiments. Biomarkers were measured using Elecsys CSF assays.
Amyloid beta (Aβ) levels varied by tube type, using a low-bind FBT reduced variation. Aβ levels were higher with no mixing versus roller/inversion mixing. Aβ levels were lower with horizontal versus upright transportation; this was resolved by maximal tube filling and storage at 2°C to 8°C. Aβ levels were less strongly affected. Phospho-tau and total-tau levels were largely unaffected.
We propose an easy-to-use, standardized, routine-use pre-analytical protocol, using low-bind FBTs, for measuring AD CSF biomarkers in clinical practice.
我们旨在建立一种标准化的、常规使用的分析前方案，用于测量脑脊液（CSF）中的阿尔茨海默病（AD）生物标志物。
使用新鲜采集的脑脊液评估分析前因素（样本采集/处理/储存/运输）对生物标志物水平的影响。使用先前冷冻的样本评估试管类型/灭菌情况。除试管类型/灭菌实验外，所有实验均使用低结合假底试管（FBT，赛多利斯公司）。使用罗氏电化学发光免疫分析法测定生物标志物。
淀粉样蛋白β（Aβ）水平因试管类型而异，使用低结合FBT可减少变异性。不混合时Aβ水平高于滚转/颠倒混合时。水平运输时Aβ水平低于直立运输时；通过最大程度地装满试管并在2°C至8°C下储存可解决此问题。Aβ水平受影响较小。磷酸化tau蛋白和总tau蛋白水平基本不受影响。
我们提出了一种易于使用、标准化的常规分析前方案，在临床实践中使用低结合FBT来测量AD脑脊液生物标志物。
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