Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, Seibersdorf, Vienna, Austria.
Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
Philos Trans R Soc Lond B Biol Sci. 2021 Feb 15;376(1818):20190808. doi: 10.1098/rstb.2019.0808. Epub 2020 Dec 28.
is the primary vector of arthropod-borne viruses including dengue, chikungunya and Zika. Vector population control methods are reviving to impede disease transmission. An efficient sex separation for male-only releases is crucial for area-wide mosquito population suppression strategies. Here, we report on the construction of two genetic sexing strains using red- and white-eye colour mutations as selectable markers. Quality control analysis showed that the Red-eye genetic sexing strains (GSS) is better and more genetically stable than the White-eye GSS. The introduction of an irradiation-induced inversion (Inv35) increases genetic stability and reduces the probability of female contamination of the male release batches. Bi-weekly releases of irradiated males of both the Red-eye GSS and the Red-eye GSS/Inv35 fully suppressed target laboratory cage populations within six and nine weeks, respectively. An image analysis algorithm allowing sex determination based on eye colour identification at the pupal stage was developed. The next step is to automate the Red-eye-based genetic sexing and validate it in pilot trials prior to its integration in large-scale population suppression programmes. This article is part of the theme issue 'Novel control strategies for mosquito-borne diseases'.
是包括登革热、基孔肯雅热和寨卡病毒在内的节肢动物传播病毒的主要载体。媒介种群控制方法正在复苏，以阻碍疾病传播。有效的雄性分离对于区域范围的蚊子种群抑制策略至关重要。在这里，我们报告了使用红眼和白眼颜色突变作为可选择标记构建两种遗传性别分离株。质量控制分析表明，红眼遗传性别分离株（GSS）比白眼 GSS 更好、更具遗传稳定性。引入辐照诱导的倒位（Inv35）增加了遗传稳定性，并降低了雄性释放批次中雌性污染的概率。每周两次释放的两种红眼 GSS 和红眼 GSS/Inv35 雄性个体在六周和九周内分别完全抑制了目标实验室笼种群。开发了一种基于眼睛颜色识别的图像分析算法，可在蛹期进行性别鉴定。下一步是在大规模种群抑制计划中整合之前，实现基于红眼的遗传性别分离的自动化，并在试点试验中对其进行验证。本文是主题为“蚊媒疾病的新控制策略”的一部分。
