Wolfson Institute for Biomedical Research and Department of Neuroscience, Physiology and Pharmacology, University College London, London, UK.
Wolfson Institute for Biomedical Research and Department of Neuroscience, Physiology and Pharmacology, University College London, London, UK.
Cell Rep. 2020 Dec 22;33(12):108537. doi: 10.1016/j.celrep.2020.108537.
Cerebellar neurons can signal sensory and motor events, but their role in active sensorimotor processing remains unclear. We record and manipulate Purkinje cell activity during a task that requires mice to rapidly discriminate between multisensory and unisensory stimuli before motor initiation. Neuropixels recordings show that both sensory stimuli and motor initiation are represented by short-latency simple spikes. Optogenetic manipulation of short-latency simple spikes abolishes or delays motor initiation in a rate-dependent manner, indicating a role in motor initiation and its timing. Two-photon calcium imaging reveals task-related coherence of complex spikes organized into conserved alternating parasagittal stripes. The coherence of sensory-evoked complex spikes increases with learning and correlates with enhanced temporal precision of motor initiation. These results suggest that both simple spikes and complex spikes govern sensory-driven motor initiation: simple spikes modulate its latency, and complex spikes refine its temporal precision, providing specific cellular substrates for cerebellar sensorimotor control.
小脑神经元可以传递感觉和运动事件的信号，但它们在主动感觉运动处理中的作用仍不清楚。在一项任务中，我们记录并操纵浦肯野细胞的活动，要求小鼠在运动开始前快速区分多感觉和单感觉刺激。神经像素记录显示，感觉刺激和运动启动都是由短潜伏期简单峰来表示的。光遗传学操纵短潜伏期简单峰以依赖于速率的方式消除或延迟运动启动，表明其在运动启动及其时间方面的作用。双光子钙成像显示，复杂峰被组织成保守的交替矢状条纹，具有任务相关的相干性。感觉诱发的复杂峰的相干性随着学习而增加，并与运动启动的时间精度增强相关。这些结果表明，简单峰和复杂峰都控制着感觉驱动的运动启动：简单峰调节其潜伏期，复杂峰则提高其时间精度，为小脑感觉运动控制提供了特定的细胞基础。
