Department of Food Science & Technology, Institute of Food Safety & Nutrition, Jinan University, Huangpu Ave. 601, Guangzhou 510632, China; Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Open Laboratory of Applied Microbiology, Guangdong Academy of Sciences, Xianlie Zhong Road 100 #, 58th Building, Guangzhou, 510070, China; College of Life Sciences, Jinan University, Guangzhou, China.
Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Open Laboratory of Applied Microbiology, Guangdong Academy of Sciences, Xianlie Zhong Road 100 #, 58th Building, Guangzhou, 510070, China.
Virus Res. 2021 Feb;293:198274. doi: 10.1016/j.virusres.2020.198274. Epub 2020 Dec 24.
Phage therapy has been revitalized since antibiotic resistance in bacteria is increasing. Compared with antibiotics, phages can target specific bacteria precisely, which requires more understanding of phage-host interactions by investigating different phages. Escherichia coli is a common pathogen with very high diversity. Based on the O antigens, E. coli can be classified into at least 183 serotypes and existing phages are far from being able to lyse all E. coli. Therefore, a novel phage specific to E. coli, named DY1, was identified and characterized to enhance our understanding of the phages of E. coli and expand the phage library. Phage DY1 belongs to the family Autographiviridae which is derived from Podoviridae. The genome of DY1 was determined to be 39,817 bp and comprises 54 putative open reading frames. Comparative genome and phylogenetic analysis demonstrated that DY1 was highly similar to phages belonging to the genus Kayfunavirus; however, the highest average nucleotide identity (ANI) values of DY1 with known phages was 0.82 suggesting that DY1 was a novel phage. Through stability tests, DY1 was very stable at temperatures ranging from 20 to 50 °C and pH levels from 5 to 11. Taken together, we report that phage DY1 is a novel Kayfunavirus phage with the potential for phage therapy.
噬菌体疗法自细菌对抗生素的耐药性不断增加以来已经复兴。与抗生素相比，噬菌体可以精确地靶向特定的细菌，这需要通过研究不同的噬菌体来更深入地了解噬菌体-宿主相互作用。大肠杆菌是一种具有非常高多样性的常见病原体。根据 O 抗原，大肠杆菌可分为至少 183 个血清型，而现有的噬菌体远不能裂解所有的大肠杆菌。因此，鉴定并表征了一种针对大肠杆菌的新型噬菌体 DY1，以增强我们对大肠杆菌噬菌体的理解并扩展噬菌体文库。噬菌体 DY1 属于 Autographiviridae 科，源自 Podoviridae 科。确定 DY1 的基因组为 39817bp，包含 54 个推定的开放阅读框。比较基因组和系统发育分析表明，DY1 与属于 Kayfunavirus 属的噬菌体高度相似；然而，DY1 与已知噬菌体的平均核苷酸同一性 (ANI) 值最高为 0.82，表明 DY1 是一种新型噬菌体。通过稳定性测试，DY1 在 20 至 50°C 的温度范围和 5 至 11 的 pH 水平下非常稳定。综上所述，我们报告称噬菌体 DY1 是一种具有噬菌体治疗潜力的新型 Kayfunavirus 噬菌体。
