姬松茸菌丝体磷酸化多糖对腺嘌呤诱导的慢性肾衰竭小鼠的抗氧化、抗炎和抗纤维化作用。
Antioxidation, anti-inflammation and anti-fibrosis effect of phosphorylated polysaccharides from Pleurotus djamor mycelia on adenine-induced chronic renal failure mice.
机构信息
College of Life Science, Shandong Agricultural University, Taian 271018, China.
Tai'an Academy of Agricultural Sciences, 271000 Tai'an, China.
出版信息
Int J Biol Macromol. 2021 Feb 15;170:652-663. doi: 10.1016/j.ijbiomac.2020.12.159. Epub 2021 Jan 5.
The mycelia polysaccharides (MPS) from Pleurotus djamor were prepared and purified by anion exchange column chromatography, and the phosphate content of phosphorylated MPS (PMPS) was 15.22 ± 0.37%. FT-IR spectra, HPLC and H and C-NMR results showed the PMPS contained α-pyranose structure and the peak area percentage composition of galacturonic acid and glucose were 13.01% and 85.82%, respectively. Animal experiment investigated the antioxidant, anti-inflammation, anti-fibrosis effects of PMPS on kidney in adenine-induced chronic renal failure (CRF) mice. All results including serum biochemical indices, histopathological observation, qRT-PCR, western blotting, immunohistochemical staining manifested the kidney injury could be remitted by PMPS interventions. This experiment suggested that PMPS could remit CRF and other kidney injury related diseases as one kind of dietary supplements and functional foods without toxic side effects.
从美味侧耳中提取并纯化的菌丝体多糖（MPS），经阴离子交换柱层析法分离纯化，得到的磷酸化 MPS（PMPS）的磷含量为 15.22±0.37%。FT-IR 光谱、HPLC 和 1H-NMR、13C-NMR 结果表明 PMPS 含有α-吡喃糖结构，半乳糖醛酸和葡萄糖的峰面积百分比组成分别为 13.01%和 85.82%。动物实验研究了 PMPS 对腺嘌呤诱导的慢性肾衰竭（CRF）小鼠肾脏的抗氧化、抗炎、抗纤维化作用。血清生化指标、组织病理学观察、qRT-PCR、western blot、免疫组织化学染色等结果均表明，PMPS 干预可减轻肾脏损伤。该实验提示 PMPS 可作为一种膳食补充剂和功能性食品，用于缓解 CRF 和其他与肾脏损伤相关的疾病，且无毒性副作用。
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