灵芝乙酰化菌丝体多糖的抗氧化与抗衰老作用。

The Antioxidant and Anti-Aging Effects of Acetylated Mycelia Polysaccharides from .

机构信息

Dezhou Academy of Agricultural Sciences, Dezhou 253015, China.

College of Life Science, Shandong Agricultural University, Taian 271018, China.

出版信息

Molecules. 2019 Jul 24;24(15):2698. doi: 10.3390/molecules24152698.

The present work mainly describes the preparation of acetylated mycelia polysaccharides (AMPS) from and investigates the antioxidant and anti-aging effects in d-galactose-induced aging mice. The optimized procedure indicates the acetyl substitution degree of AMPS is 0.54 ± 0.04 under the conditions of a reaction time of 56 h, a reaction temperature of 37 °C, and 4 mL of added acetic anhydride. The in vitro analysis and in vivo animal experiments indicate that the AMPS could alleviate the aging properties by scavenging the radicals, elevating the enzyme activities, and reducing the lipid contents. As for serum levels, the AMPS can improve the serum biochemical indices and enhance immunological activity. The histopathological observations indicate that the injuries to the liver, kidney, and brain can be remitted by AMPS intervention. The characterization showed that AMPS was one kind of β-pyranose with the weight-average molecular weights of 3.61 × 10 Da and the major monosaccharides of mannose and glucose. The results suggest that AMPS can be used as a dietary supplement and functional food for the prevention of aging and age-related diseases.

本工作主要描述了从中制备乙酰化菌丝体多糖 (AMPS)，并研究了其在半乳糖诱导衰老小鼠中的抗氧化和抗衰老作用。优化后的工艺条件为：反应时间 56 h、反应温度 37°C、添加 4 mL 乙酸酐，在此条件下 AMPS 的乙酰取代度为 0.54±0.04。体外分析和体内动物实验表明，AMPS 可以通过清除自由基、提高酶活性和降低脂质含量来减轻衰老特性。就血清水平而言，AMPS 可以改善血清生化指标并增强免疫活性。组织病理学观察表明，AMPS 干预可以减轻肝、肾和脑的损伤。表征结果表明，AMPS 是一种β-吡喃糖，重均分子量为 3.61×10 Da，主要单糖为甘露糖和葡萄糖。结果表明，AMPS 可用作预防衰老和与衰老相关疾病的膳食补充剂和功能性食品。