National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214000, PR China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214000, PR China.
National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214000, PR China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214000, PR China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214000, PR China.
Bioresour Technol. 2021 Mar;323:124554. doi: 10.1016/j.biortech.2020.124554. Epub 2020 Dec 17.
The removal efficacy of biological nitrogen removal process is inhibited by low temperatures. Herein, a psychrotrophic bacterium strain, Bacillus simplex H-b, was isolated and identified with the potential to conduct heterotrophic nitrification and aerobic denitrification in the temperature range from 5 to 37 °C. At 10 °C, the removal efficiencies of initial nitrate-N (63 mg/L), nitrite-N (10 mg/L) and ammonium-N (60 mg/L) were 67.29%, 78.69% and 82.16%, with the maximum removal rate of 0.56, 0.18 and 0.74 mg/L/h, respectively. Additionally, both the accumulation level of ATP (adenosine triphosphate) and the formation of extracellular polymeric substances was found to increase with the decrease of temperature from 37 °C to 10 °C, indicating strain H-b might resist low temperature stress through its cellular extreme environment resistant mechanism and further suggesting the newly isolated strain could serve as a promising candidate for nitrogen contaminated wastewater treatment, especially under low-temperature condition.
生物脱氮过程的去除效果会受到低温的抑制。本文从 5 到 37°C 的温度范围内分离和鉴定出了一株嗜冷菌，即简单芽孢杆菌 H-b，该菌具有异养硝化和好氧反硝化的能力。在 10°C 时，初始硝酸盐-N（63mg/L）、亚硝酸盐-N（10mg/L）和氨氮-N（60mg/L）的去除效率分别为 67.29%、78.69%和 82.16%，最大去除速率分别为 0.56、0.18 和 0.74mg/L/h。此外，随着温度从 37°C 降低到 10°C，ATP（三磷酸腺苷）的积累水平和胞外聚合物物质的形成均有所增加，表明菌株 H-b 可能通过其细胞极端环境抗性机制来抵抗低温胁迫，进一步表明新分离的菌株可作为处理含氮废水的有前途的候选物，特别是在低温条件下。
