Post-Graduate Program in Adult Health, Federal University of Maranhão - UFMA, São Luís, Brazil.
Post-Graduate Program in Adult Health, Basic and Applied Immunology Nucleum (NIBA/DEPAT), Federal University of Maranhão - UFMA, São Luís, Brazil.
PLoS One. 2020 Dec 23;15(12):e0228828. doi: 10.1371/journal.pone.0228828. eCollection 2020.
This study evaluated the effect of green propolis extract on the adhesion and biofilm formation of Candida species in dentistry materials. Phytochemical analysis of green propolis extract was performed by high-performance liquid chromatography. Adhesion was quantified by counting the number of yeast cells adherent to dental material fragments in a Neubauer chamber. Biofilm formation was determined by counting colony-forming units recovered from dental material fragments. The intensity of biofilm adhesion was classified as negative, weak, moderate, strong, or very strong. Fifteen compounds, mainly flavonoids, were identified in green propolis extract. All strains adhered to and formed biofilms on the surfaces of the orthodontic materials studied. On steel and resin, yeast cell adhesion intensities were weak at all incubation times, except for those of Candida parapsilosis and C. tropicalis, which were moderate at 12 h. At 24 and 48 h, C. albicans formed biofilms on steel with moderate adhesion affinities; at 24 and 48 h, C. parapsilosis formed biofilms with very strong affinities. C. tropicalis formed biofilms with strong and very strong affinities at 24 and 48 h, respectively. On resin, all species displayed strong affinity for biofilm formation at 24 and 48 h, except for C. tropicalis, which displayed very strong affinity at only 48 h. Green propolis extract displayed antifungal activity and inhibited both adhesion and biofilm formation at 2.5 μg/mL. This study reinforces the idea that green propolis has antifungal activity and interferes with the virulence of Candida species.
本研究评估了绿色蜂胶提取物对牙科材料中念珠菌黏附和生物膜形成的影响。采用高效液相色谱法对绿色蜂胶提取物进行了植物化学成分分析。通过纽巴氏室计数黏附在牙科材料碎片上的酵母细胞数量来定量评估黏附作用。通过从牙科材料碎片中回收的菌落形成单位来确定生物膜形成情况。生物膜黏附强度分为阴性、弱阳性、中度、强阳性或极强阳性。在绿色蜂胶提取物中鉴定出了 15 种化合物，主要为类黄酮。所有菌株均在研究的正畸材料表面黏附和形成生物膜。在钢和树脂上，除了近平滑念珠菌和热带念珠菌在 12 小时时为中度外，所有酵母细胞的黏附强度均为弱阳性。在 24 和 48 小时时，白色念珠菌在钢片上形成具有中度黏附亲和力的生物膜；在 24 和 48 小时时，近平滑念珠菌形成具有极强黏附亲和力的生物膜。热带念珠菌在 24 和 48 小时时分别形成具有强和极强亲和力的生物膜。在树脂上，除了热带念珠菌仅在 48 小时时具有极强亲和力外，所有物种在 24 和 48 小时时均显示出强烈的生物膜形成亲和力。绿色蜂胶提取物在 2.5μg/mL 时显示出抗真菌活性，并抑制黏附和生物膜形成。本研究进一步证实了绿色蜂胶具有抗真菌活性并干扰念珠菌属物种的毒力这一观点。
