Wu Qiulin, Yin Guobing, Lei Jinwei, Tian Jiao, Lan Ailin, Liu Shengchun
Department of Endocrine and Breast Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Department of Breast and Thyroid Surgery, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Front Mol Biosci. 2020 Dec 10;7:599110. doi: 10.3389/fmolb.2020.599110. eCollection 2020.
KLHL5 (Kelch Like Family Member 5) is differentially expressed in gastric cancer, but its correlation with prognosis and functioning mechanism in gastric cancer remain unclear. The Oncomine database and TIMER were employed to appraise the KLHL5 expression in a variety of cancers. The correlation between KLHL5 expression and patient prognosis was extracted from the Kaplan-Meier plotter, GEPIA, and PrognoScan database. Then the relationship between KLHL5 expression and inflammatory infiltrate profiles was inquired by TIMER. Finally, GEPIA and TIMER were explored for the correlative significance between KLHL5 expression and immune cell-related marker sets. KLHL5 was found to be differentially expressed and correlated with clinical outcomes in several types of cancers in the TCGA database. Especially, KLHL5 mRNA expression was upregulated and correlated with poorer overall survival and progression-free survival in gastric cancer. Moreover, elevated KLHL5 expression was significantly related with patient node stage, infiltration level, and expression of multiple immune marker sets. These results implicate that KLHL5 expression is closely linked with patient clinical outcomes and the microenvironmental infiltration level in different neoplasms. This indicates that KLHL5 is a modulator in infiltrate recruitment, shaping the landscape of immune cell infiltration. Thus, it represents an eligible prognostic predictor for gastric malignancy.
KLHL5（kelch样家族成员5）在胃癌中存在差异表达，但其与胃癌预后的相关性及作用机制仍不清楚。利用Oncomine数据库和TIMER评估KLHL5在多种癌症中的表达。从Kaplan-Meier Plotter、GEPIA和PrognoScan数据库中提取KLHL5表达与患者预后的相关性。然后通过TIMER探究KLHL5表达与炎症浸润特征之间的关系。最后，利用GEPIA和TIMER研究KLHL5表达与免疫细胞相关标志物集之间的相关意义。研究发现，在TCGA数据库中，KLHL5在几种癌症中存在差异表达并与临床结果相关。特别是，KLHL5 mRNA表达上调，且与胃癌患者较差的总生存期和无进展生存期相关。此外，KLHL5表达升高与患者的淋巴结分期、浸润水平以及多种免疫标志物集的表达显著相关。这些结果表明，KLHL5表达与不同肿瘤患者的临床结果及微环境浸润水平密切相关。这表明KLHL5是浸润招募的调节因子，塑造了免疫细胞浸润的格局。因此，它是胃癌恶性肿瘤的一个合格的预后预测指标。
