Kong Xiaoxiao, Fu Meili, Niu Xing, Jiang Hongxing
Department of General Surgery, Linyi People's Hospital Affiliated to Shandong University, Linyi, China.
Department of Infectious Diseases, Linyi People's Hospital Affiliated to Shandong University, Linyi, China.
Front Oncol. 2020 Sep 4;10:1086. doi: 10.3389/fonc.2020.01086. eCollection 2020.
TIM-1 is a critical gene that regulates T-helper cell development. However, little research has revealed the distribution, prognosis, and immune infiltration of TIM-1 in cancers. TCGA, GEO, Oncomine, TIMER, Kaplan-Meier, PrognoScan, GEPIA, TISIDB, and HPA databases were used to analyze TIM-1 in cancers. High TIM-1 expression was observed in bladder, cholangio, head and neck, colorectal, gastric, kidney, liver, lung adenocarcinoma, skin, uterine corpus endometrial, and pancreatic cancers compared to the normal tissues, and immunofluorescence shows that TIM-1 is mainly localized in vesicles. Simultaneously, high TIM-1 expression was closely related with poorer overall survival in gastric, lung adenocarcinoma, and poorer disease-specific survival in gastric cancer in the TCGA cohort, and was validated in the GEO cohort. Moreover, high expression of TIM-1, correlated with clinical relevance of gastric cancer and lung adenocarcinoma, was associated with tumor-infiltrating lymphocytes in lung adenocarcinoma and gastric cancer. Finally, immunohistochemistry showed TIM-1 expression was higher in lung adenocarcinoma and gastric cancer compared to the normal tissues. In summary, we applied integrated bioinformatics approaches to suggest that TIM-1 can be used as a prognostic biomarker in gastric and lung adenocarcinoma, which might provide a novel direction to explore the pathogenesis of gastric and lung adenocarcinoma.
TIM-1是一个调节辅助性T细胞发育的关键基因。然而，关于TIM-1在癌症中的分布、预后及免疫浸润情况的研究较少。本研究利用TCGA、GEO、Oncomine、TIMER、Kaplan-Meier、PrognoScan、GEPIA、TISIDB和HPA数据库对癌症中的TIM-1进行分析。与正常组织相比，在膀胱癌、胆管癌、头颈癌、结直肠癌、胃癌、肾癌、肝癌、肺腺癌、皮肤癌、子宫内膜癌和胰腺癌中观察到TIM-1高表达，免疫荧光显示TIM-1主要定位于囊泡中。同时，在TCGA队列中，TIM-1高表达与胃癌、肺腺癌患者较差的总生存期密切相关，与胃癌患者较差的疾病特异性生存期密切相关，且在GEO队列中得到验证。此外，TIM-1高表达与胃癌和肺腺癌的临床相关性相关，与肺腺癌和胃癌中的肿瘤浸润淋巴细胞有关。最后，免疫组化显示与正常组织相比，肺腺癌和胃癌中TIM-1表达更高。总之，我们应用综合生物信息学方法表明，TIM-1可作为胃癌和肺腺癌的预后生物标志物，这可能为探索胃癌和肺腺癌的发病机制提供新方向。
