Gopi Marappan, Manojkumar Villavan, Verma Ashok Kumar, Singh Putan, Rokade Jaydip Jaywant, Pearlin Beulah V, Monika Madheswaran, Madhupriya Velusamy, SaravanaKumar Manimaran, Tamilmani Tamilselvan
Division of Avian Physiology and Reproduction, Central Avian Research Institute, Uttar Pradesh, India.
Division of Animal Nutrition, Indian Veterinary Research Institute, Uttar Pradesh, India.
Front Vet Sci. 2020 Dec 11;7:583748. doi: 10.3389/fvets.2020.583748. eCollection 2020.
An study on the effect of the administration of a combination of nucleosides (25, 50, and 100 mg/egg) on hatchability, growth performance, energy metabolizability, and intestinal morphology in broilers was carried out. Four hundred eighty (480) fertile eggs were divided into four groups (in four replicates each having 30 eggs). On the 18th days of incubation of the eggs, candling was carried out and the fertile eggs were selected and given one of the four administrations. Group one served as control and was injected with phosphate-buffered saline (PBS). The other groups were given administration of nucleosides (25, 50, and 100 mg/egg) at 100 μl through the yolk sac route, and chicks of respective groups were hatched out. Among the experimental groups, the hatchability was comparable; however, the hatchability was affected in the group injected with a higher level of nucleosides at 100 mg/egg. The hatched out chicks from higher doses of nucleosides (50 and 100 mg) had higher body weight (BW) ( < 0.05) than the control. Higher energy metabolizability (%) was observed in nucleoside-injected groups. Plasma protein concentration was higher in groups administered with nucleosides (50 and 100 mg). Histologically, the intestinal villi length was maximum in 100 mg-injected group followed by 50 and 25 mg. Relative expression of homeobox (Cdx) in the jejunum was significantly ( < 0.05) upregulated in all the injected groups at 3, 7, and 14 days of age. Nucleoside-administered groups had better performance, energy metabolizability, and intestinal morphology. Among the experimental groups, the administration of nucleosides at 50 mg/egg resulted in higher growth performance, plasma protein, intestinal surface, and villi development in broiler chickens.
进行了一项关于核苷组合（25、50和100毫克/枚鸡蛋）给药对肉鸡孵化率、生长性能、能量代谢率和肠道形态影响的研究。480枚受精蛋被分为四组（每组四个重复，每组30枚蛋）。在蛋孵化的第18天，进行照蛋，挑选出受精蛋并给予四种处理之一。第一组作为对照组，注射磷酸盐缓冲盐水（PBS）。其他组通过卵黄囊途径以100微升的量给予核苷（25、50和100毫克/枚鸡蛋）处理，各组成雏鸡孵化出壳。在实验组中，孵化率相当；然而，注射100毫克/枚鸡蛋较高水平核苷的组孵化率受到影响。较高剂量核苷（50和100毫克）孵化出的雏鸡体重（BW）高于对照组（P<0.05）。核苷注射组观察到较高的能量代谢率（%）。给予核苷（50和100毫克）的组血浆蛋白浓度较高。组织学上，100毫克注射组的肠道绒毛长度最长，其次是50毫克和25毫克组。在3、7和14日龄时，所有注射组空肠中同源框（Cdx）的相对表达均显著上调（P<0.05）。核苷给药组具有更好的性能、能量代谢率和肠道形态。在实验组中，以50毫克/枚鸡蛋的剂量给予核苷可使肉鸡具有更高的生长性能、血浆蛋白、肠道表面积和绒毛发育。
