Hallböök F, Ebendal T, Persson H
Department of Medical Genetics, Uppsala University, Sweden.
Mol Cell Biol. 1988 Jan;8(1):452-6. doi: 10.1128/mcb.8.1.452-456.1988.
DNA fragments encoding either rat or chicken beta nerve growth factor (NGF) were inserted in the expression vector p91023(B) for transient expression in COS cells. The two NGF constructs produced RNA transcripts and proteins of the predicted sizes. Conditioned media from the transfected cells stimulated neurite outgrowth from cultured chicken embryo sympathetic ganglia. The results show that the rat or chicken NGF gene can direct the synthesis of a biologically active NGF protein after transfection of COS cells.
将编码大鼠或鸡β神经生长因子（NGF）的DNA片段插入表达载体p91023(B)，以便在COS细胞中进行瞬时表达。这两种NGF构建体产生了预测大小的RNA转录本和蛋白质。来自转染细胞的条件培养基刺激了培养的鸡胚交感神经节的神经突生长。结果表明，大鼠或鸡NGF基因在转染COS细胞后能够指导合成具有生物活性的NGF蛋白。
