Waris Muhammad, Nasir Shabab, Rasule Azhar, Yousaf Iqra
Department of Zoology, Government College University, Faisalabad, Pakistan.
J Arthropod Borne Dis. 2020 Jun 30;14(2):162-172. doi: 10.18502/jad.v14i2.3734. eCollection 2020 Jun.
mosquitoes are the most important group of vectors having ability of transmitting pathogens including arboviruses that can cause serious diseases like Chikungunya fever, Dengue fever and Zika virus in human. Biosynthesis and the use of green silver nanoparticles (AgNPs) is an important step in the search of reliable and ecofriendly control of these vectors.
In this study an aqueous leaves extract of (castor) and silver nanoparticles (AgNPs) synthesized from this extract were evaluated as larvicidal agent for 2 and 3 instar larvae of the . Different concentrations (50, 100, 150, 200 and 250ppm) of plant extract and synthesized nanoparticles were prepared and applied on second and third instar larvae. The percent mortality was noted after 6, 12, 18, 24, 30, 36, 42 and 48H of exposure and subjected to probit analysis to calculate LC and LC.
Synthesized Ag nanoparticles were characterized by UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FT-IR), and energy-dispersive X-ray spectroscopy (XRD). The nanoparticles were more toxic against larvae of with LC value (49.43ppm) and LC value (93.65ppm) for 2 instar larvae and LC (84.98ppm) and LC (163.89ppm) for 3 instar larvae as compared to the plant extract (149.58ppm, 268.93ppm) and (155.58ppm, 279.93ppm) for 2 and 3 instar larvae of respectively after 48H.
Our results suggest the extract of and synthesized nanoparticles as excellent replacement of chemical pesticides to control the vector mosquitoes.
蚊子是最重要的病媒群体，能够传播包括虫媒病毒在内的病原体，这些病毒可在人类中引发基孔肯雅热、登革热和寨卡病毒等严重疾病。绿色银纳米颗粒（AgNPs）的生物合成及其应用是寻求可靠且环保的病媒控制方法的重要一步。
在本研究中，对蓖麻的水提叶提取物以及由该提取物合成的银纳米颗粒（AgNPs）作为致倦库蚊二龄和三龄幼虫的杀幼虫剂进行了评估。制备了不同浓度（50、100、150、200和250ppm）的植物提取物和合成纳米颗粒，并将其应用于二龄和三龄幼虫。在暴露6、12、18、24、30、36、42和48小时后记录死亡率，并进行概率分析以计算LC50和LC90。
通过紫外可见光谱、傅里叶变换红外光谱（FT-IR）和能量色散X射线光谱（XRD）对合成的银纳米颗粒进行了表征。与植物提取物相比，纳米颗粒对致倦库蚊幼虫的毒性更大，48小时后，二龄幼虫的LC50值（49.43ppm）和LC90值（93.65ppm），三龄幼虫的LC50（84.98ppm）和LC90（163.89ppm），而二龄和三龄致倦库蚊幼虫的植物提取物LC50分别为（149.58ppm，268.93ppm）和LC90（155.58ppm，279.93ppm）。
我们的结果表明，蓖麻提取物和合成纳米颗粒是控制病媒蚊子的化学杀虫剂的极佳替代品。
