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(豆科)野大豆(Willd.)Ohwi的叶绿体全基因组序列:一种传统中草药。

Complete chloroplast genome sequence of (Willd.) Ohwi (Fabaceae): a traditional Chinese medicinal herb.

作者信息

Sun Jiahui, Wang Ling, Yuan Yuan, Zhou Rongrong, Duan Haiyan, Zhao Yuping

机构信息

National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.

出版信息

Mitochondrial DNA B Resour. 2019 Dec 9;5(1):25-26. doi: 10.1080/23802359.2019.1694850.

DOI:10.1080/23802359.2019.1694850
PMID:33366405
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7720969/
Abstract

is a traditional Chinese herb which has been used medically since ancient times. In this study, we sequenced the complete chloroplast genome of based on next-generation sequencing and used the data to assess genomic resources. The chloroplast genome of is 153,411 bp in length consisting of large and small single-copy regions of length 84,142 and 17,989 bp, separated by two IR regions of 25,640 bp. The overall GC content was 35.4%. assembly and annotation showed the presence of 112 unique genes with 78 protein-coding genes, 30 tRNA genes, and four rRNA genes. A maximum-likelihood phylogenomic analysis showed that was sister to .

摘要

是一种自古以来就被用于医学的传统中草药。在本研究中,我们基于二代测序对其叶绿体全基因组进行了测序,并利用这些数据评估基因组资源。其叶绿体基因组长度为153,411 bp,由长度分别为84,142和17,989 bp的大单拷贝区和小单拷贝区组成,这两个区域被两个长度为25,640 bp的反向重复区隔开。总体GC含量为35.4%。组装和注释显示存在112个独特基因,其中包括78个蛋白质编码基因、30个tRNA基因和4个rRNA基因。最大似然系统发育基因组分析表明,与 是姐妹关系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6926/7720969/1f0f91f7cab2/TMDN_A_1694850_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6926/7720969/1f0f91f7cab2/TMDN_A_1694850_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6926/7720969/1f0f91f7cab2/TMDN_A_1694850_F0001_B.jpg

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本文引用的文献

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PeerJ. 2016 Nov 10;4:e2699. doi: 10.7717/peerj.2699. eCollection 2016.
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Plann: A command-line application for annotating plastome sequences.Plann:一个用于注释质体基因组序列的命令行应用程序。
Appl Plant Sci. 2015 Aug 10;3(8). doi: 10.3732/apps.1500026. eCollection 2015 Aug.
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RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies.RAxML 版本 8:用于系统发育分析和大型系统发育后分析的工具。
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Genome Biol Evol. 2013;5(5):989-97. doi: 10.1093/gbe/evt063.
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SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing.SPAdes:一种新的基因组组装算法及其在单细胞测序中的应用
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