Wu Liuyu, Wu Xuming, Xiong Xiao, Xin Tianrong, Wang Yayu, Zou Zhiwen, Xia Bin
College of Life Science, Nanchang University, Nanchang, China.
Plant Protection Station, County of Dayu, Ganzhou, China.
Mitochondrial DNA B Resour. 2016 Jul 23;1(1):532-533. doi: 10.1080/23802359.2016.1197065.
The complete mitochondrial genome of (GenBank accession number KU870700) was sequenced by traditional PCR amplification and primer walking methods. The total length was 15,368 bp, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a A + T-rich region. The base composition of the genome was A (40.85%), T (39.34%), C (12.01%) and G (7.8%), respectively. The arrangement of all genes was identical to other lepidopteran insects. The phylogenetic relationships were established based on the nucleotide sequences of 13 protein-coding genes of mitochondrial genomes by the neighbor-joining method. The molecular-based phylogeny supported the traditional morphological classification on relationships within Lepidoptera species.
通过传统的PCR扩增和引物步移法对（GenBank登录号KU870700）的完整线粒体基因组进行了测序。其全长为15368bp，包括13个蛋白质编码基因、2个核糖体RNA基因、22个转移RNA基因和一个富含A+T的区域。基因组的碱基组成为A（40.85%）、T（39.34%）、C（12.01%）和G（7.8%）。所有基因的排列与其他鳞翅目昆虫相同。基于线粒体基因组13个蛋白质编码基因的核苷酸序列，采用邻接法建立了系统发育关系。基于分子的系统发育支持了鳞翅目物种间关系的传统形态学分类。
