Reza Md Sajib Al, Hasan Md Mahmudul, Kamruzzaman Md, Hossain Md Imam, Zubair Md Abu, Bari Luthfunnesa, Abedin Md Zainul, Reza Md Abu, Khalid-Bin-Ferdaus Khandaker Md, Haque Kazi Md Faisal, Islam Khairul, Ahmed Mahtab Uddin, Hossain Md Khaled
Department of Food Technology and Nutritional Science Faculty of Life Science Mawlana Bhashani Science and Technology University Tangail Bangladesh.
Protein Science Lab Department of Genetic Engineering and Biotechnology Faculty of Science University of Rajshahi Rajshahi Bangladesh.
Food Sci Nutr. 2019 Jan 29;7(2):667-677. doi: 10.1002/fsn3.906. eCollection 2019 Feb.
This study was conducted to evaluate the toxic effects of an azo dye carmoisine widely used in foods and to investigate its relation to carcinogenicity. Carmoisine administered into mice orally in four different doses as control, low, medium, and high equivalent to 0, 4, 200, and 400 mg/kg bw, respectively, for 120 days. The key toxicological endpoint was observed including animal body weight, organ weights, hematology, biochemistry, and molecular biology assessment. The body weights of medium- and high-dose carmoisine-treated mice group were significantly decreased as compared to the control mice group. Platelet, white blood cell and monocyte counts of treated group were considerably higher, while Hb and red blood cell counts were drastically lower than the control group. The biochemical parameters such as serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total protein, globulin, urea, and creatinine level were significantly increased, while serum cholesterol level was decreased after treatment as compared to the control. RT-PCR results showed that expression of Bcl-x and PARP gene was intensively increased, whereas expression of p gene was decreased in the mouse liver tissues treated with carmoisine. This study revealed that high-dose (400 mg/kg bw) treatment of carmoisine was attributable to renal failure and hepatotoxicity. It also would be suspected as a culprit for liver oncogenesis.
本研究旨在评估一种广泛用于食品的偶氮染料胭脂红的毒性作用，并研究其与致癌性的关系。分别以相当于0、4、200和400 mg/kg体重的四种不同剂量给小鼠口服胭脂红，持续120天。观察了关键的毒理学终点，包括动物体重、器官重量、血液学、生物化学和分子生物学评估。与对照小鼠组相比，中、高剂量胭脂红处理的小鼠组体重显著下降。处理组的血小板、白细胞和单核细胞计数显著高于对照组，而血红蛋白和红细胞计数则显著低于对照组。与对照组相比，处理后血清丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶、总蛋白、球蛋白、尿素和肌酐水平等生化参数显著升高，而血清胆固醇水平降低。RT-PCR结果显示，在经胭脂红处理的小鼠肝组织中，Bcl-x和PARP基因的表达显著增加，而p基因的表达则降低。本研究表明，高剂量（400 mg/kg体重）的胭脂红处理可导致肾衰竭和肝毒性。它也可能被怀疑是肝脏肿瘤发生的罪魁祸首。
