Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore 117543, Singapore.
Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore.
Plant Commun. 2020 Feb 19;1(2):100033. doi: 10.1016/j.xplc.2020.100033. eCollection 2020 Mar 9.
Nuclear pore complexes (NPCs), which comprise multiple copies of nucleoporins (Nups), are large protein assemblies embedded in the nuclear envelope connecting the nucleus and cytoplasm. Although it has been known that Nups affect flowering in , the underlying mechanisms are poorly understood. Here, we show that loss of function of () leads to increased abundance of CONSTANS (CO) protein and the resulting upregulation of () specifically in the morning. We demonstrate that Nup160 regulates CO protein stability through affecting NPC localization of an E3-ubiquitin ligase, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1 (HOS1), which destabilizes CO protein in the morning period. Taken together, these results provide a mechanistic understanding of Nup function in the transition from vegetative to reproductive growth, suggesting that deposition of HOS1 at NPCs by Nup160 is essential for preventing precocious flowering in response to photoperiod in .
核孔复合体（NPC）由多个核孔蛋白（Nups）组成，是嵌入核膜中的大型蛋白复合物，连接细胞核和细胞质。尽管已知 Nups 影响开花时间，但背后的机制尚不清楚。在这里，我们表明功能丧失导致 CONSTANS（CO）蛋白丰度增加，并且在早晨特异性地上调 （）。我们证明 Nup160 通过影响 E3-泛素连接酶 HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1（HOS1）的 NPC 定位来调节 CO 蛋白稳定性，该酶在早晨使 CO 蛋白不稳定。总之，这些结果提供了 NPC 功能在从营养生长到生殖生长过渡中的机制理解，表明由 Nup160 在 NPC 上沉积 HOS1 对于防止拟南芥对光周期的过早开花反应是必不可少的。
