National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.
Plant Commun. 2020 Jun 20;1(4):100088. doi: 10.1016/j.xplc.2020.100088. eCollection 2020 Jul 13.
Plants have developed various mechanisms for avoiding pathogen invasion, including () genes. Most genes encode nucleotide-binding domain and leucine-rich repeat containing proteins (NLRs). Here, we report the isolation of three new bacterial blight genes in rice, , , and (), which were allelic to and encoded atypical NLRs with unique central tandem repeats (CTRs). We also found that () was the same gene as . Although and conferred different resistance spectra, their performance could be attenuated by iTALEs, as has previously been reported for . XA1, XA1-2, XA14, and non-resistant RGAF differed mainly in the substructure of the leucine-rich repeat domain. They all contained unique CTRs and belonged to the CTR-NLRs, which existed only in Gramineae. We also found that interactions among these genes led to differing resistance performance. In conclusion, our results uncover a unique locus in rice consisting of at least three multiple alleles (, , and ) that encode CTR-NLRs and confer resistance to pv. ().
植物已经开发出各种机制来避免病原体入侵，包括 () 基因。大多数基因编码核苷酸结合域和富含亮氨酸重复的蛋白（NLRs）。在这里，我们报道了在水稻中分离到三个新的细菌性条斑病基因、、和（），它们是与和等位的，编码具有独特中央串联重复（CTR）的非典型 NLRs。我们还发现（）与相同。虽然和赋予不同的抗性谱，但它们的性能可以像以前报道的那样被 iTALEs 减弱。XA1、XA1-2、XA14 和非抗性 RGAF 主要在富含亮氨酸重复区的亚结构上有所不同。它们都含有独特的 CTRs，属于仅存在于禾本科中的 CTR-NLRs。我们还发现这些基因之间的相互作用导致了不同的抗性表现。总之，我们的结果揭示了水稻中至少由三个复等位基因（、和）组成的一个独特基因座，这些基因编码 CTR-NLRs，对 pv. （）具有抗性。
Zotero 插件
