Neurosciences, Pharmacology and Environment Team, Laboratory of Clinical, Experimental and Environmental Neurosciences, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, Morocco; Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh, Morocco.
Epidemiology and Biomedical Sciences Unit, Laboratory of Health Sciences and Technologies, Higher Institute of Health Sciences, Hassan First University of Settat, Settat, Morocco.
J Chem Neuroanat. 2021 Mar;112:101915. doi: 10.1016/j.jchemneu.2020.101915. Epub 2020 Dec 25.
Aluminum (Al) is recognized potent neurotoxic metal, which causes oxidative stress leading to intracellular accumulation of reactive oxygen species (ROS) and neuronal cell death in various neurodegenerative diseases. Among several medicinal plants with beneficial effects on health, curcumin acts as a multi-functional drug with antioxidant activity. Thus, the purpose of the present study was to evaluate the protective effect of curcumin against aluminum induced-oxidative stress and astrocytes death, in vitro ad in vivo. Incubation of cultured rat astrocytes with two concentrations of Al (37 μM and 150 μM) for 1 h provoked a dose-dependent reduction of the number of living cells as evaluated by Fluorescein diacetate and lactate dehydrogenase assay. Al-treated cells exhibited a reduction of both superoxide dismutase (SOD) and catalase activities. Pretreatment of astrocytes with curcumin (81 μM) prevented Al-induced cell death. Regarding in vivo study, rats were exposed acutely during three consecutive days to three different doses of Al (25 mg/kg, 50 mg/kg and 100 mg/kg, i.p injection), together with curcumin treatment (30 mg/kg). For the chronic model, animals were exposed to Al (3 g/l) in drinking water from intrauterine age to 4 months ages, plus curcumin treatment (175 mg/kg). Data showed that both acute and chronic Al intoxication induced an obvious astrogliosis within motor cortex and hippocampus, while, such effects were restored by curcumin. We showed herein that Al was highly toxic, induced astrocytes death. Then, curcumin protected astrocytes against Al-toxicity. The cytoprotective potential of curcumin is initiated by stimulation of endogenous antioxidant system.
铝（Al）是一种公认的强效神经毒性金属，它会导致氧化应激，从而导致各种神经退行性疾病中活性氧（ROS）的细胞内积累和神经元细胞死亡。在具有有益健康作用的几种药用植物中，姜黄素具有抗氧化活性的多功能药物作用。因此，本研究的目的是评估姜黄素对铝诱导的氧化应激和星形胶质细胞死亡的体外和体内保护作用。将两种浓度的 Al（37 μM 和 150 μM）孵育在培养的大鼠星形胶质细胞中 1 小时，通过荧光素二乙酸酯和乳酸脱氢酶测定评估活细胞数量呈剂量依赖性减少。Al 处理的细胞表现出超氧化物歧化酶（SOD）和过氧化氢酶活性的降低。姜黄素（81 μM）预处理可防止 Al 诱导的细胞死亡。关于体内研究，大鼠在连续三天内分别接受三种不同剂量的 Al（25 mg/kg、50 mg/kg 和 100 mg/kg，腹腔注射）和姜黄素治疗（30 mg/kg）。对于慢性模型，动物从宫内年龄到 4 月龄暴露于饮用水中的 Al（3 g/l），同时进行姜黄素治疗（175 mg/kg）。数据显示，急性和慢性 Al 中毒都会引起运动皮层和海马体中明显的星形胶质增生，而姜黄素则恢复了这种作用。我们在此表明，Al 具有高度毒性，会诱导星形胶质细胞死亡。然后，姜黄素可保护星形胶质细胞免受 Al 的毒性。姜黄素的细胞保护潜力是通过刺激内源性抗氧化系统启动的。
