Wu Wenbin, Zhang Yangmei, Li Xiaowu, Wang Xiang, Yuan Yuan
Department of Thoracic Surgery, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, 221009, China.
Department of Medical Oncology, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, 221009, China.
Curr Gene Ther. 2021;21(4):290-298. doi: 10.2174/1566523220666201229155833.
The purpose of this study was to explore the mechanism of the miR-375/XPR1 axis in esophageal squamous cell carcinoma (ESCC) and provide a new idea for targeted therapy of ESCC.
Differentially expressed genes in GEO and TCGA databases were analyzed by bioinformatics. The expression levels of miR-375 and XPR1 mRNA were detected by qRT-PCR. Protein expression of XPR1 was detected by western blot. Bioinformatics analysis and dual luciferase assay were conducted to confirm the target relationship between miR-375 and XPR1. The viability, proliferation, migration and invasion of cells in each treatment group were detected by CCK-8, colony formation, wound healing and Transwell assays.
Significantly down-regulated miR-375 and remarkably up-regulated XPR1 were observed in ESCC tissue and cells. Overexpression of miR-375 inhibited proliferation, invasion and migration of ESCC cells, and greatly reduced the promoting effect of XPR1 overexpression on cell proliferation, migration and invasion. Dual luciferase assay confirmed that miR-375 targeted and inhibited XPR1 expression in ESCC.
These results demonstrate the regulatory role of the miR-375/XPR1 axis in ESCC cells and provide a new potential target for the precise treatment of patients with ESCC.
本研究旨在探讨miR-375/XPR1轴在食管鳞状细胞癌（ESCC）中的作用机制，为ESCC的靶向治疗提供新思路。
通过生物信息学分析GEO和TCGA数据库中的差异表达基因。采用qRT-PCR检测miR-375和XPR1 mRNA的表达水平。通过蛋白质印迹法检测XPR1的蛋白表达。进行生物信息学分析和双荧光素酶报告基因检测以确认miR-375与XPR1之间的靶向关系。采用CCK-8、集落形成、伤口愈合和Transwell实验检测各处理组细胞的活力、增殖、迁移和侵袭能力。
在ESCC组织和细胞中观察到miR-375显著下调，XPR1显著上调。miR-375过表达抑制了ESCC细胞的增殖、侵袭和迁移，并大大降低了XPR1过表达对细胞增殖、迁移和侵袭的促进作用。双荧光素酶报告基因检测证实miR-375在ESCC中靶向并抑制XPR1的表达。
这些结果证明了miR-375/XPR1轴在ESCC细胞中的调控作用，并为ESCC患者的精准治疗提供了新的潜在靶点。
