LeBrun Erick S, Nighot Meghali, Dharmaprakash Viszwapriya, Kumar Anand, Lo Chien-Chi, Chain Patrick S G, Ma Thomas Y
Biosecurity and Public Health, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Department of Medicine, Division of Gastroenterology and Hepatology, Penn State College of Medicine, Hershey, PA 17033, USA.
Life (Basel). 2020 Dec 24;11(1):7. doi: 10.3390/life11010007.
Phenotypic health effects, both positive and negative, have been well studied in association with the consumption of alcohol in humans as well as several other mammals including mice. Many studies have also associated these same health effects and phenotypes to specific members of gut microbiome communities. Here we utilized a chronic plus binge ethanol feed model (Gao-binge model) to explore microbiome community changes across three independent experiments performed in mice. We found significant and reproducible differences in microbiome community assemblies between ethanol-treated mice and control mice on the same diet absent of ethanol. We also identified significant differences in gut microbiota occurring temporally with ethanol treatment. Peak shift in communities was observed 4 days after the start of daily alcohol consumption. We quantitatively identified many of the bacterial genera indicative of these ethanol-induced shifts including 20 significant genera when comparing ethanol treatments with controls and 14 significant genera based on temporal investigation. Including overlap of treatment with temporal shifts, we identified 25 specific genera of interest in ethanol treatment microbiome shifts. Shifts coincide with observed presentation of fatty deposits in the liver tissue, i.e., Alcoholic Liver Disease-associated phenotype. The evidence presented herein, derived from three independent experiments, points to the existence of a common, reproducible, and characterizable "mouse ethanol gut microbiome".
关于人类以及包括小鼠在内的其他几种哺乳动物饮酒所产生的表型健康影响，无论是积极的还是消极的，都已经得到了充分研究。许多研究还将这些相同的健康影响和表型与肠道微生物群落的特定成员联系起来。在这里，我们利用慢性加暴饮乙醇喂养模型（高暴饮模型），在小鼠身上进行的三个独立实验中探索微生物群落的变化。我们发现在相同不含乙醇的饮食条件下，乙醇处理的小鼠和对照小鼠之间的微生物群落组装存在显著且可重复的差异。我们还确定了乙醇处理过程中肠道微生物群随时间出现的显著差异。在开始每日饮酒4天后观察到群落的峰值转移。我们定量鉴定了许多指示这些乙醇诱导变化的细菌属，在将乙醇处理组与对照组比较时，有20个显著属，基于时间调查有14个显著属。包括处理与时间变化的重叠，我们确定了乙醇处理微生物群落变化中25个特定的感兴趣属。这些变化与在肝组织中观察到的脂肪沉积表现一致，即酒精性肝病相关表型。本文所提供的证据来自三个独立实验，表明存在一个共同的、可重复的且可表征 的“小鼠乙醇肠道微生物群”。
