Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Biology, College of Science, Qassim University, Qassim 51452, Saudi Arabia.
Molecules. 2020 Dec 23;26(1):29. doi: 10.3390/molecules26010029.
Seven endophytic fungi were isolated from the tropical medicinal plant L. After preliminary screening, was selected for the epigenetic activation treatments. The antibacterial, antifungal, and antioxidant potentials of crude extracts obtained from the treatments (with and without epigenetic modifiers) were analyzed in vitro. The extracts inhibited growth of the human pathogens , and , as well as the phytopathogens , and . Furthermore, DPPH-scavenging activity was higher in valproic acid treated extracts. Volatile chemicals with known biological activities (measured with GC-MS/MS), were released in the valproic acid treatment. The antimicrobial potentials of the extracts were confirmed using MRM/MS analysis. The experiments revealed a new promising endophytic fungus, , to be utilized in biological plant protection and in biomedical applications.
从热带药用植物 L. 中分离出 7 株内生真菌。经过初步筛选，选择 用于表观遗传激活处理。分析了处理（有和没有表观遗传修饰剂）获得的粗提物的体外抗菌、抗真菌和抗氧化潜力。提取物抑制了人类病原体 和 的生长，以及植物病原体 和 。此外，用丙戊酸处理的提取物的 DPPH 清除活性更高。用 GC-MS/MS 测量，丙戊酸处理释放出具有已知生物活性的挥发性化学物质。使用 MRM/MS 分析证实了提取物的抗菌潜力。实验揭示了一种有前途的新内生真菌 ，可用于生物植物保护和生物医学应用。
