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利用短读长和长读长测序方法对大豆中常见夜蛾抗性基因周围的基因组序列数据进行表征。

Characterization of the genomic sequence data around common cutworm resistance genes in soybean () using short- and long-read sequencing methods.

作者信息

Ogiso-Tanaka Eri, Oki Nobuhiko, Tanaka Tsuyoshi, Shimizu Takehiko, Ishimoto Masao, Hajika Makita, Kaga Akito

机构信息

Institute of Crop Science, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.

Kyushu Okinawa Agricultural Research Center, NARO, 2421 Suya, Koushi, Kumamoto 861-1192, Japan.

出版信息

Data Brief. 2020 Dec 9;34:106577. doi: 10.1016/j.dib.2020.106577. eCollection 2021 Feb.

DOI:10.1016/j.dib.2020.106577
PMID:33376760
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7758510/
Abstract

The common cutworm (CCW, Fabricius) is one of the pests that most severely infect soybean ( L. Merr.). In a previous report, quantitative trait loci (QTL) analysis of CCW resistance using a recombinant inbred line derived from a cross between a susceptible cultivar 'Fukuyutaka' and a resistant cultivar 'Himeshirazu', identified two antixenosis resistance QTLs, and . To reveal sequence variation between the aforementioned two cultivars, whole genome resequencing was performed using Illumina HiSeq2000 (75,632,747 and 91,540,849 reads). The generated datasets can be used for fine mapping and gene isolation of and as well as for revealing more detailed genetic differences between 'Fukuyutaka' and 'Himeshirazu' .

摘要

小地老虎(CCW,法布里丘斯)是对大豆(L. Merr.)危害最严重的害虫之一。在之前的一份报告中,利用感病品种‘福余丰’和抗病品种‘姬白绢’杂交得到的重组自交系,对小地老虎抗性进行数量性状位点(QTL)分析,鉴定出两个抗生性QTL,即 和 。为揭示上述两个品种之间的序列变异,使用Illumina HiSeq2000进行了全基因组重测序(分别获得75,632,747条和91,540,849条 reads)。生成的数据集可用于 和 的精细定位和基因分离,以及揭示‘福余丰’和‘姬白绢’之间更详细的遗传差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ceb/7758510/656958b7bba6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ceb/7758510/20e6c74d713a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ceb/7758510/1422b24ea6f5/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ceb/7758510/656958b7bba6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ceb/7758510/20e6c74d713a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ceb/7758510/1422b24ea6f5/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ceb/7758510/656958b7bba6/gr3.jpg

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