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基于微载体的规模化生产和纯化人诱导多能干细胞源性神经元和星形胶质细胞的方案。

A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytes.

机构信息

School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA.

School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA; Graduate Program in Clinical Translational Sciences, University of Arizona College of Medicine-Phoenix, Phoenix, AZ 85004, USA.

出版信息

STAR Protoc. 2022 Aug 18;3(3):101632. doi: 10.1016/j.xpro.2022.101632. eCollection 2022 Sep 16.

DOI:10.1016/j.xpro.2022.101632
PMID:36035791
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9405537/
Abstract

Here, we describe a protocol for a microcarrier (MC)-based, large-scale generation and cryopreservation of human-induced pluripotent stem cell (hiPSC)-derived neurons and astrocytes. We also detail steps to isolate these populations with a high degree of purity. Finally, we describe how to cryopreserve these cell types while maintaining high levels of viability and preserving cellular function post-thaw. For complete details on the use and execution of this protocol, please refer to Brookhouser et al. (2021).

摘要

在此，我们描述了一种基于微载体 (MC) 的大规模生成和冷冻保存人诱导多能干细胞 (hiPSC) 衍生神经元和星形胶质细胞的方案。我们还详细介绍了如何以高纯度分离这些细胞群的步骤。最后，我们描述了如何在冷冻保存这些细胞类型的同时保持高存活率并在解冻后保持细胞功能。有关该方案的使用和执行的完整详细信息，请参阅 Brookhouser 等人（2021 年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/6b40e5e9b2ac/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/0312e723bebe/fx1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/f5623ca223a2/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/6b88b0ad2ad9/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/ac5cb6b20a79/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/4ce25c7bf22e/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/c3de4671959a/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/5d1297b41c94/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/6b40e5e9b2ac/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/0312e723bebe/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/6a2cb68b9d7f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/f5623ca223a2/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/6b88b0ad2ad9/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/ac5cb6b20a79/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/4ce25c7bf22e/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/c3de4671959a/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/5d1297b41c94/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/63d3/9405537/6b40e5e9b2ac/gr8.jpg

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本文引用的文献

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