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从种子中提取的精油和纯露用于控制[具体内容缺失]和[具体内容缺失]。

The Use of Essential Oil and Hydrosol Extracted from Seeds for the Control of and .

作者信息

Pardavella Iro, Daferera Demetra, Tselios Theodoros, Skiada Panagiota, Giannakou Ioannis

机构信息

Laboratory of Agricultural Zoology and Entomology, Department of Science of Crop Production, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.

Laboratory of Chemistry, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.

出版信息

Plants (Basel). 2020 Dec 28;10(1):46. doi: 10.3390/plants10010046.

Abstract

The essential oil (EO) and hydrosol (HL) isolated from (cumin) seeds were evaluated against the root-knot nematodes and . The efficacy of extracts on the motility, hatching, and survival in soil of second-stage juveniles (J2s), and the activity on egg differentiation were tested. All J2s were paralyzed after immersion in the EO at 62.5 μL/L concentration for 96 h. Encouraging results were recorded using HL equal to or higher than 10% concentration for both species tested. More than 70% paralyzed J2s were recorded after immersion for 48 h, while the percentage was increased to higher than 90% after 96 h of immersion. A clear effect on egg differentiation was observed after immersion in EO or HL. A significant decrease in egg differentiation was revealed at even low concentrations of EO while an evident decrease in egg differentiation was recorded after immersion of eggs in 50% HL dilution. Decreased hatching of and J2s was observed with the increase in concentration. The lowest numbers of hatched J2s were recorded when EO was used at 1000 and 2000 μL/L concentrations. A constant reduction in root-knot nematode J2 hatching was observed upon increasing the concentration of HL from 5% to 50%. The EO of is characterized by the presence of γ-terpinene-7-al (34.95%), cumin aldehydes (26.48), and α-terpinene-7-al (12.77%). The above constituents were observed in HL following the same order as that observed in EO. The components γ-terpinene (11.09%) and -cymene (6.56%) were also recorded in EO while they were absent in HL.

摘要

对从小茴香种子中分离得到的精油(EO)和纯露(HL)进行了抗根结线虫[Meloidogyne incognita(Kofoid & White)Chitwood]和南方根结线虫[Meloidogyne arenaria(Neal)Chitwood]的评估。测试了提取物对二龄幼虫(J2s)在土壤中的运动性、孵化率和存活率的影响,以及对卵分化的活性。所有J2s在62.5μL/L浓度的EO中浸泡96小时后均麻痹。对于所测试的两种线虫,使用浓度等于或高于10%的HL时记录到了令人鼓舞的结果。浸泡48小时后记录到超过70%的J2s麻痹,而浸泡96小时后该百分比增加到高于90%。浸泡在EO或HL中后观察到对卵分化有明显影响。即使在低浓度的EO下也显示出卵分化显著降低,而将卵浸泡在50%HL稀释液中后记录到卵分化明显降低。随着浓度增加,观察到南方根结线虫和爪哇根结线虫J2s的孵化率降低。当EO以1000和2000μL/L浓度使用时,记录到的孵化J2s数量最少。随着HL浓度从5%增加到50%,观察到根结线虫J2s孵化率持续降低。小茴香的EO的特征在于含有γ-萜品烯-7-醛(占34.95%)、小茴香醛(占26.48%)和α-萜品烯-7-醛(占12.77%)。在HL中观察到上述成分,其顺序与在EO中观察到的相同。成分γ-萜品烯(占11.09%)和对异丙基甲苯(占6.56%)也在EO中记录到,而在HL中不存在。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21fe/7823439/3ee506e1605b/plants-10-00046-g001.jpg

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