Spedicato Massimo, Compagni Emiliano Delli, Caporale Marialuigia, Teodori Liana, Leone Alessandra, Ancora Massimo, Mangone Iolanda, Perletta Fabrizia, Portanti Ottavio, Di Giallonardo Francesca, Bonfini Barbara, Savini Giovanni, Lorusso Alessio
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZS-Teramo), Teramo, Italy.
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise (IZS-Teramo), Teramo, Italy.
Res Vet Sci. 2022 Dec 10;151:36-41. doi: 10.1016/j.rvsc.2022.07.003. Epub 2022 Jul 10.
Bluetongue virus (BTV) is the etiologic agent of bluetongue, a WOAH (founded as Office International des Épizooties, OIE)-notifiable economically important disease of ruminants. BTV is transmitted by Culicoides biting midges and 24 different "classical" serotypes have been reported to date. In recent years, several putative novel BTV serotypes, often referred to as "atypical" BTVs, have been documented. These are characterized by unusual biological characteristics, most notably avirulence and vector-independent transmission. Here, we describe the recurrence of such an atypical virus strain BTV-X ITL2021 detected in goats six years after its first discovery in Sardinia, Italy. Combined serological and genome analysis results clearly suggest that the two strains belong to the same BTV serotype. However, unlike the 2015 strain, BTV-X ITL2021 was successfully isolated in BSR cell-culture allowing further serological characterization. Lastly, seropositivity for BTV-X ITL2021 was detected by virus-neutralization in approximately 74% of animals tested, suggesting that this atypical BTV serotype has been circulating undetected in asymptomatic animals for years.
蓝舌病病毒（BTV）是蓝舌病的病原体，蓝舌病是一种世界动物卫生组织（前身为国际兽疫局，OIE）规定须通报的、对反刍动物具有重要经济影响的疾病。BTV通过库蠓叮咬传播，迄今为止已报告有24种不同的“经典”血清型。近年来，已记录到几种推定的新型BTV血清型，通常被称为“非典型”BTV。这些病毒具有不同寻常的生物学特性，最显著的是无毒力和不依赖媒介传播。在此，我们描述了这样一种非典型病毒株BTV-X ITL2021在意大利撒丁岛首次发现六年后在山羊中再次出现的情况。血清学和基因组分析结果综合表明，这两种毒株属于同一BTV血清型。然而，与2015年的毒株不同，BTV-X ITL2021在BSR细胞培养中成功分离，从而能够进行进一步的血清学鉴定。最后，通过病毒中和试验在约74%的受试动物中检测到BTV-X ITL2021的血清阳性，这表明这种非典型BTV血清型已在无症状动物中隐匿传播多年。
