Department of Nutrition and Food Safety, West China School of Public Health, West China Fourth Hospital, Sichuan University, Chengdu, Sichuan, China.
Division II of Risk Surveillance, NHC Key Laboratory of Food Safety Risk Assessment, Food Safety Research Unit (2019RU014) of Chinese Academy of Medical Science; China National Center for Food Safety Risk Assessment, Beijing, China.
Foodborne Pathog Dis. 2023 Apr;20(4):158-168. doi: 10.1089/fpd.2022.0040.
Invasive listeriosis is a rare but serious foodborne disease that causes maternal-neonatal, central nervous system, and bloodstream infections. The aim of this study was to assess the whole-genome sequencing (WGS)-based genetic diversity of clinical isolates over a 7-year period and prove the effect of WGS application in food vehicle investigation. A total of 360 isolates were recovered during 2013 and 2019 through the national listeriosis special surveillance program. Two hundred twenty-six isolates (62.8%) were associated with pregnancy. All isolates belonged to lineage I (214 isolates) or lineage II (146 isolates), with 4 serogroups (46.9% IIb, 39.7% IIa, 12.5% IVb, and 0.8% IIc). All isolates were in 25 clonal complexes (CCs) and 3 singletons, with CC87, CC8, and CC5 being the most common causes of human listeriosis. All clinical isolates were positive for pathogenicity island 1 (LIPI-1), LIPI-3 was present in 21.4% of isolates and LIPI-4 was detected in 29.2% of isolates. LIPI-4-positive isolates, including CC87, sequence type (ST)619, ST382, CC4, and CC2, have been shown to confer hypervirulence. Fifteen isolates harbored at least one antimicrobial encoding gene, including (M), (A), (D), and (G). The sublineage designations were consistent with CC designations, and 215 distinct cgMLST types (CTs) were classified, the most abundant being CT58 and CT750. In summary, there is a high level of genetic diversity among the clinical isolates. WGS has strengthened listeriosis surveillance and will be implemented for other foodborne bacteria in the National Molecular Tracing Network for Foodborne Disease.
侵袭性李斯特菌病是一种罕见但严重的食源性疾病，可导致母婴、中枢神经系统和血流感染。本研究旨在评估 7 年间临床分离株基于全基因组测序（WGS）的遗传多样性，并证明 WGS 应用于食品传播媒介调查的效果。通过国家李斯特菌专项监测计划，于 2013 年至 2019 年共分离出 360 株菌。226 株（62.8%）与妊娠有关。所有分离株均属于谱系 I（214 株）或谱系 II（146 株），有 4 个血清群（46.9% IIb、39.7% IIa、12.5% IVb 和 0.8% IIc）。所有分离株分属于 25 个克隆复合体（CC）和 3 个单克隆体，CC87、CC8 和 CC5 是人类李斯特菌病最常见的原因。所有临床分离株均为致病性岛 1（LIPI-1）阳性，21.4%的分离株存在 LIPI-3，29.2%的分离株存在 LIPI-4。LIPI-4 阳性分离株，包括 CC87、序列型（ST）619、ST382、CC4 和 CC2，已被证明具有超毒力。15 株分离株至少携带一种编码抗生素的基因，包括 （M）、 （A）、 （D）和 （G）。亚谱系命名与 CC 命名一致，共分为 215 种不同的 cgMLST 型（CT），其中最丰富的是 CT58 和 CT750。总之，临床分离株具有高度的遗传多样性。WGS 加强了李斯特菌病监测，将在国家食源性疾病分子溯源网络中用于其他食源性细菌。
