Research institute of bioscience and biotechnology, University of Tabriz, Tabriz, Iran.
Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
J Fluoresc. 2021 Jul;31(4):1191-1202. doi: 10.1007/s10895-021-02726-z. Epub 2021 May 26.
In this work, novel Fe/ZnO nanocomposites (NCs) and Fe nanoparticles loaded onto porous ZnO nanostructures have been synthesized via a simple biotechnological route by using Berberis thunbergii extract. In this direction, the as-synthesized bio-based porous ZnO derivatives and human serum albumin (HSA), as a biopolymeric model, form nano-hybrid assemblies. The effect of loading Fe on properties of porous ZnO nanostructures as well as the behavior of the nano-hybrid assemblies were evaluated by using XRD, SEM, EDX, DLS, PL, CD, FTIR and UV/Visible-diffuse reflectance spectra (UV/Vis-DRS) techniques. The fluorescence results revealed that the interaction of Fe/ZnO NCs with HSA biopolymer led to the formation of a ground state complexes as nano-hybrid assemblies. The calculated thermodynamic parameters indicated that the binding process occurred spontaneously. The CD and FTIR spectra confirmed the changes in helicity of HSA as well as the random coil and β-turn in the secondary structure of HSA upon interaction with Fe/ZnO NCs.
在这项工作中，通过使用小檗属植物提取物，通过简单的生物技术路线合成了新型的 Fe/ZnO 纳米复合材料 (NCs) 和负载在多孔 ZnO 纳米结构上的 Fe 纳米粒子。在这个方向上，合成的基于生物的多孔 ZnO 衍生物和人血清白蛋白 (HSA) 作为生物聚合模型，形成纳米杂化组装体。通过使用 XRD、SEM、EDX、DLS、PL、CD、FTIR 和 UV/可见漫反射光谱 (UV/Vis-DRS) 技术评估了负载 Fe 对多孔 ZnO 纳米结构的性能以及纳米杂化组装体的行为的影响。荧光结果表明，Fe/ZnO NCs 与 HSA 生物聚合物的相互作用导致形成了基态复合物作为纳米杂化组装体。计算的热力学参数表明，结合过程是自发发生的。CD 和 FTIR 光谱证实了在与 Fe/ZnO NCs 相互作用时 HSA 的螺旋性以及 HSA 二级结构中的无规卷曲和β-转角的变化。
