Functional Foods, Nutraceuticals and Phytomedicine Laboratory, Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
Graduate Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Center of Natural and Exacts Sciences, Federal University of Santa Maria, Santa Maria, RS, 97105-900, Brazil.
Metab Brain Dis. 2021 Apr;36(4):627-638. doi: 10.1007/s11011-020-00659-3. Epub 2021 Jan 4.
Microglia are immune cells that are resident in central nervous system. Activation of microglial cells are detrimental to the survival of neurons. Thus, prevention of microglia activation and/or protection against microglia activation could be potential therapeutic strategy towards the management of inflammation-mediated neurodegenerative diseases. Moringa oleifera is widely consumed as food and used in folklore medicine for treating several diseases. This study was convened to investigate the effect of aqueous extract of Moringa oleifera on cell viability, cholinergic and purinergic enzymes in BV-2 microglial cultured cell. Aqueous extract of Moringa oleifera was prepared, lyophilized and reconstituted in 0.5% dimethylsulphoxide (DMSO). Cells were treated with Moringa oleifera extracts (0.1-100 μg/mL) and assessed for cell viability and nitric oxide production. Furthermore, the effect of Moringa oleifera on enzymes of cholinergic (acetylcholinesterase) and purinergic (nucleoside triphosphate diphosphohydrolase; NTPDase, 5' nucleotidase and adenosine deaminase; ADA) systems in BV-2 microglial cells were determined. Incubation of BV-2 microglia cell with M. oleifera extract maintained cell viability, modulated cholinergic and purinergic enzymes activity. The phenolic compounds found in M. oleifera extracts, include chlorogenic acid, rutin; quercetin pentoside, kaempferol derivative and quercetin derivative. Thus, this study suggest that the potential therapeutic effect of the phenolic compounds found in M. oleifera may have been responsible for the maintenance of cell viability in BV-2 microglia cells and modulation of cholinergic as well as purinergic enzymes activity.
小胶质细胞是驻留在中枢神经系统中的免疫细胞。小胶质细胞的激活对神经元的存活有害。因此，预防小胶质细胞的激活和/或防止小胶质细胞的激活可能是管理炎症介导的神经退行性疾病的潜在治疗策略。辣木被广泛用作食物，并在民间医学中用于治疗多种疾病。本研究旨在探讨辣木叶的水提取物对 BV-2 小胶质细胞培养细胞活力、胆碱能和嘌呤能酶的影响。制备辣木叶的水提取物，冷冻干燥并在 0.5%二甲基亚砜 (DMSO) 中重新配制。用辣木叶提取物（0.1-100μg/mL）处理细胞，并评估细胞活力和一氧化氮产生。此外，还确定了辣木叶对 BV-2 小胶质细胞中胆碱能（乙酰胆碱酯酶）和嘌呤能（核苷三磷酸二磷酸水解酶；NTPDase、5'核苷酸酶和腺苷脱氨酶；ADA）系统酶的影响。用辣木叶提取物孵育 BV-2 小胶质细胞可维持细胞活力，调节胆碱能和嘌呤能酶的活性。辣木叶提取物中发现的酚类化合物包括绿原酸、芦丁；槲皮素戊糖苷、山奈酚衍生物和槲皮素衍生物。因此，本研究表明，辣木叶中发现的酚类化合物的潜在治疗作用可能负责维持 BV-2 小胶质细胞的细胞活力，并调节胆碱能和嘌呤能酶的活性。
