Department of Plastic, Reconstructive and Aesthetic Surgery, Acıbadem University Faculty of Medicine, İstanbul-Turkey.
Department of Plastic, Reconstructive and Aesthetic Surgery, Necmettin Erbakan University Meram Faculty of Medicine, Konya-Turkey.
Ulus Travma Acil Cerrahi Derg. 2021 Jul;27(4):395-401. doi: 10.14744/tjtes.2020.12247.
The present study aims to investigate the favorable effects of melatonin on burn wound healing in rats.
In this study, forty Wistar-albino-type male rats were divided into four groups. Group 1 was the control group, Group 2 rats were treated using exogenous melatonin, Group 3 rats were pinealectomized, and Group 4 rats were pinealectomized then treated with exogenous melatonin. In all groups, a deep second-degree burn was created on the backs of the rats with a metal plate heated in boiling water. We monitored the progress of burn healing for seven days. At the end of them, we evaluated hydroxyproline levels, type III collagen, edema, inflammatory infiltration, congestion, vascular proliferation, fibrosis, the thickness of the zone of stasis and the epithelium to assess the progress of healing.
The zone of stasis was less thick in Group 2 than the other groups (p=0.009). Type III collagen dyeing (p=0.031), fibrosis (p=0.011) and edema (p=0.031) were higher in Group 2 than the other groups. Congestion was higher in the control group than Group 4 (p=0.031). Other evaluated parameters showed no significant differences among the groups.
In this study, it was noted that once total melatonin levels exceeded a certain threshold, a preventive effect was exerted on burn wound damage progression by reducing the zone of stasis. Melatonin may also prevent the development of hypertrophic scarring. Melatonin may be a potential therapeutic option that can supplement traditional treatment in burn wounds; however, further studies with higher doses of exogenous melatonin administered over longer periods are needed to further evaluate the effects noted in this study.
本研究旨在探讨褪黑素对大鼠烧伤创面愈合的有益作用。
本研究将 40 只 Wistar 白化雄性大鼠分为四组。第 1 组为对照组，第 2 组大鼠给予外源性褪黑素治疗，第 3 组大鼠行松果体切除术，第 4 组大鼠行松果体切除术并给予外源性褪黑素治疗。在所有组中，使用在沸水中加热的金属板在大鼠背部造成深二度烧伤。我们监测了 7 天的烧伤愈合进展。在结束时，我们评估了羟脯氨酸水平、III 型胶原、水肿、炎症浸润、充血、血管增生、纤维化、淤血带厚度和上皮，以评估愈合进展。
第 2 组的淤血带厚度比其他组薄（p=0.009）。第 2 组 III 型胶原染色（p=0.031）、纤维化（p=0.011）和水肿（p=0.031）均高于其他组。对照组的充血程度高于第 4 组（p=0.031）。其他评估参数在组间无显著差异。
在这项研究中，我们注意到一旦总褪黑素水平超过一定阈值，通过减少淤血带，褪黑素对烧伤创面损伤进展就会产生预防作用。褪黑素还可以预防增生性瘢痕的形成。褪黑素可能是一种有潜力的治疗选择，可以补充烧伤创面的传统治疗方法；然而，需要进行更高剂量的外源性褪黑素给药更长时间的进一步研究，以进一步评估本研究中观察到的效果。
