Yang Hua, Bayer Philipp E, Tirnaz Soodeh, Edwards David, Batley Jacqueline
School of Biological Sciences, University of Western Australia, Crawley, WA 6009, Australia.
School of Agriculture and Food Sciences, University of Queensland, St Lucia, QLD 4067, Australia.
Biology (Basel). 2020 Dec 30;10(1):17. doi: 10.3390/biology10010017.
, an allotetraploid species, is an important germplasm resource for canola improvement, due to its many beneficial agronomic traits, such as heat and drought tolerance and blackleg resistance. Receptor-like kinase (RLK) and receptor-like protein (RLP) genes are two types of resistance gene analogues (RGA) that play important roles in plant innate immunity, stress response and various development processes. In this study, genome wide analysis of RLKs and RLPs is performed in . In total, 493 RLKs (LysM-RLKs and LRR-RLKs) and 228 RLPs (LysM-RLPs and LRR-RLPs) are identified in the genome of , using RGAugury. Only 13.54% RLKs and 11.79% RLPs are observed to be grouped within gene clusters. The majority of RLKs (90.17%) and RLPs (52.83%) are identified as duplicates, indicating that gene duplications significantly contribute to the expansion of RLK and RLP families. Comparative analysis between and its progenitor species, and , indicate that 83.62% RLKs and 41.98% RLPs are conserved in , and RLPs are likely to have a faster evolution than RLKs. This study provides a valuable resource for the identification and characterisation of candidate RLK and RLP genes.
作为一个异源四倍体物种，由于其具有许多有益的农艺性状，如耐热、耐旱和抗黑胫病，是油菜改良的重要种质资源。类受体激酶（RLK）和类受体蛋白（RLP）基因是两类抗性基因类似物（RGA），在植物先天免疫、应激反应和各种发育过程中发挥重要作用。在本研究中，对[物种名称未给出]进行了RLK和RLP的全基因组分析。使用RGAugury在[物种名称未给出]的基因组中总共鉴定出493个RLK（赖氨酸基序-RLK和富含亮氨酸重复序列-RLK）和228个RLP（赖氨酸基序-RLP和富含亮氨酸重复序列-RLP）。仅观察到13.54%的RLK和11.79%的RLP被归类在基因簇中。大多数RLK（90.17%）和RLP（52.83%）被鉴定为重复基因，表明基因重复对RLK和RLP家族的扩展有显著贡献。[物种名称未给出]与其祖先物种[物种名称未给出]和[物种名称未给出]之间的比较分析表明，83.62%的RLK和41.98%的RLP在[物种名称未给出]中是保守的，并且RLP的进化可能比RLK更快。本研究为候选RLK和RLP基因的鉴定和表征提供了有价值的资源。
