Department of Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht 41635-1314, Iran.
Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Karaj 31485-498, Iran.
Cells. 2021 Dec 23;11(1):37. doi: 10.3390/cells11010037.
The LysM receptor-like kinases (LysM-RLKs) play a crucial role in plant symbiosis and response to environmental stresses. , , and are utilized as valuable vegetables. Different biotic and abiotic stressors affect these crops, resulting in yield losses. Therefore, genome-wide analysis of the LysM-RLK gene family was conducted. From the genome of the examined species, 33 LysM-RLK have been found. The conserved domains of LysM-RLKs were divided into three groups: LYK, LYP, and LysMn. In the gene family, only segmental duplication has occurred. The Ka/Ks ratio for the duplicated pair of genes was less than one indicating that the genes' function had not changed over time. The contain 70 cis-elements, indicating that they are involved in stress response. 39 miRNA molecules were responsible for the post-transcriptional regulation of 12 . A total of 22 SSR loci were discovered in 16 . According to RNA-seq data, the highest expression in response to biotic stresses was related to BnLYP6. According to the docking simulations, several residues in the active sites of BnLYP6 are in direct contact with the docked chitin and could be useful in future studies to develop pathogen-resistant . This research reveals comprehensive information that could lead to the identification of potential genes for species genetic manipulation.
LysM 受体样激酶（LysM-RLKs）在植物共生和对环境胁迫的反应中起着至关重要的作用。芜菁、甘蓝和花椰菜被广泛用作有价值的蔬菜。不同的生物和非生物胁迫因素会影响这些作物，导致产量损失。因此，对 LysM-RLK 基因家族进行了全基因组分析。从被研究物种的基因组中，发现了 33 个 LysM-RLK。LYK、LYP 和 LysMn 这三个组被划分到 中的 LysM-RLK 的保守结构域中。在 基因家族中，只发生了片段重复。基因对的 Ka/Ks 比值小于 1，表明基因的功能没有随时间而改变。 含有 70 个顺式作用元件，表明它们参与了胁迫反应。12 个 受到 39 个 miRNA 分子的转录后调控。在 16 个 中发现了 22 个 SSR 位点。根据 RNA-seq 数据，对生物胁迫的最高表达与 BnLYP6 有关。根据对接模拟，BnLYP6 活性位点中的几个残基与对接的几丁质直接接触，这在未来的研究中可能有助于开发抗病原体的 。这项研究揭示了全面的信息，可能导致鉴定出用于 物种遗传操作的潜在基因。
