Department of Radiological Science, College of Health Science, Eulji University, Seongnam, South Korea.
Department of Radiologic Technology, Cheju Halla University, Jeju-si, Jeju-do, South Korea.
PLoS One. 2021 Apr 2;16(4):e0249530. doi: 10.1371/journal.pone.0249530. eCollection 2021.
To investigate the therapeutic effect of local photothermal (PT) heating on suppression of stent-induced granulation tissue formation in mouse colon.
A gold nanoparticle (GNP)-coated self-expandable metallic stent (SEMS) was prepared using a two-step synthesis process for local PT heating under near-infrared laser irradiation. Twenty-four mice were randomly divided into two groups of 12 and subjected to SEMS placement in the colon. Group A received a GNP-coated SEMS without local heating and Group B received a GNP-coated SEMS and underwent local heating at 55°C after SEMS placement. The therapeutic effect of local heating was assessed by comparing the histopathological, immunohistochemical, and endoscopic results.
Four mice were excluded because of stent migration (n = 3, group B) or death (n = 1, group A). Stent-induced granulation tissue-related variables were significantly lower in group B than in group A (p < 0.001). In vivo endoscopic images, 4 weeks after stent placement, showed granulation tissue formation over the wire mesh in group A and relatively good patency of the stented colon with no definite irregularities in group B. There was more vascular endothelial growth factor (VEGF) positivity in group A than in group B.
Local PT heating suppresses granulation tissue formation after stent placement in mouse colon.
研究局部光热（PT）加热对抑制小鼠结肠支架诱导的肉芽组织形成的治疗效果。
采用两步合成法制备了金纳米颗粒（GNP）涂层自膨式金属支架（SEMS），用于近红外激光照射下的局部 PT 加热。24 只小鼠随机分为两组，每组 12 只，在结肠中放置 SEMS。A 组接受未进行局部加热的 GNP 涂层 SEMS，B 组接受 GNP 涂层 SEMS，并在放置 SEMS 后进行 55°C 的局部加热。通过比较组织病理学、免疫组织化学和内镜结果评估局部加热的治疗效果。
由于支架迁移（n = 3，B 组）或死亡（n = 1，A 组），有 4 只小鼠被排除在外。B 组支架诱导的肉芽组织相关变量明显低于 A 组（p < 0.001）。支架放置 4 周后体内内镜图像显示，A 组的金属丝网上有肉芽组织形成，B 组的支架结肠保持较好的通畅性，没有明显的不规则。A 组的血管内皮生长因子（VEGF）阳性表达高于 B 组。
局部 PT 加热可抑制小鼠结肠支架放置后肉芽组织的形成。
