Department of Interventional Therapy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.
Department of Interventional Radiology, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510080, China.
Eur Radiol Exp. 2024 Apr 3;8(1):40. doi: 10.1186/s41747-024-00438-0.
To assess the feasibility and tissue response of using a gold nanoparticle (AuNP)-integrated silicone-covered self-expandable metal stent (SEMS) for local hyperthermia in a rat esophageal model.
The study involved 42 Sprague-Dawley rats. Initially, 6 animals were subjected to near-infrared (NIR) laser irradiation (power output from 0.2 to 2.4 W) to assess the in vitro heating characteristics of the AuNP-integrated SEMS immediately after its placement. The surface temperature of the stented esophagus was then measured using an infrared thermal camera before euthanizing the animals. Subsequently, the remaining 36 animals were randomly divided into 4 groups of 9 each. Groups A and B received AuNP-integrated SEMS, while groups C and D received conventional SEMS. On day 14, groups A and C underwent NIR laser irradiation at a power output of 1.6 W for 2 min. By days 15 (3 animals per group) or 28 (6 animals per group), all groups were euthanized for gross, histological, and immunohistochemical analysis.
Under NIR laser irradiation, the surface temperature of the stented esophagus quickly increased to a steady-state level. The surface temperature of the stented esophagus increased proportionally with power outputs, being 47.3 ± 1.4 °C (mean ± standard deviation) at 1.6 W. Only group A attained full circumferential heating through all layers, from the epithelium to the muscularis propria, demonstrating marked apoptosis in these layers without noticeable necroptosis.
Local hyperthermia using the AuNP-integrated silicone-covered SEMS was feasible and induced cell death through apoptosis in a rat esophageal model.
A gold nanoparticle-integrated silicone-covered self-expanding metal stent has been developed to mediate local hyperthermia. This approach holds potential for irreversibly damaging cancer cells, improving the sensitivity of cancer cells to therapies, and triggering systemic anticancer immune responses.
• A gold nanoparticle-integrated silicone-covered self-expanding metal stent was placed in the rat esophagus. • Upon near-infrared laser irradiation, this stent quickly increased the temperature of the stented esophagus. • Local hyperthermia using this stent was feasible and resulted in cell death through apoptosis.
评估在大鼠食管模型中使用金纳米颗粒（AuNP）整合的硅胶覆盖自膨式金属支架（SEMS）进行局部热疗的可行性和组织反应。
本研究纳入了 42 只 Sprague-Dawley 大鼠。最初，6 只动物接受近红外（NIR）激光照射（功率输出范围为 0.2 至 2.4 W），以评估 AuNP 整合的 SEMS 放置后立即的体外加热特性。然后，用红外热像仪测量支架食管的表面温度，然后处死动物。随后，其余 36 只动物随机分为 4 组，每组 9 只。A 组和 B 组接受 AuNP 整合的 SEMS，C 组和 D 组接受常规 SEMS。第 14 天，A 组和 C 组在功率输出为 1.6 W 的情况下接受 NIR 激光照射 2 分钟。第 15 天（每组 3 只）或第 28 天（每组 6 只），所有组均处死进行大体、组织学和免疫组织化学分析。
在近红外激光照射下，支架食管的表面温度迅速升高到稳定水平。支架食管的表面温度与功率输出成正比，在 1.6 W 时为 47.3±1.4°C（平均值±标准差）。只有 A 组通过所有层（从上皮到固有肌层）实现了完全周向加热，这些层显示出明显的细胞凋亡，但没有明显的坏死性细胞死亡。
在大鼠食管模型中，使用 AuNP 整合的硅胶覆盖的 SEMS 进行局部热疗是可行的，并通过细胞凋亡诱导细胞死亡。
已经开发出一种金纳米颗粒整合的硅胶覆盖的自膨式金属支架来介导局部热疗。这种方法有可能不可逆地破坏癌细胞，提高癌细胞对治疗的敏感性，并引发全身性抗癌免疫反应。
• 在大鼠食管中放置了一种金纳米颗粒整合的硅胶覆盖的自膨式金属支架。• 在近红外激光照射下，这种支架迅速升高了支架食管的温度。• 使用这种支架进行局部热疗是可行的，并通过细胞凋亡导致细胞死亡。
