Departments of Radiology and Research Institute of Radiology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul, 05505, Republic of Korea.
Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul, 05505, Republic of Korea.
Sci Rep. 2021 May 18;11(1):10558. doi: 10.1038/s41598-021-90182-x.
Minimally invasive therapies using stent technology are currently limited by stent-induced granulation tissue formation adjacent to the stent. The effectiveness of photothermal therapy (PTT) using a gold nanoparticle (AuNP)-coated stent for treating stent-induced granulation tissue formation in the rat esophagus was investigated. All experiments were approved by the animal research committee of our institution. An AuNP-coated, self-expandable metallic stent (SEMS) was produced to conduct PTT under near-infrared laser irradiation. Forty rats were randomly divided into four groups (10 rats each). The animals in group A (non-coated SEMS) and group B (AuNP-coated SEMS with local heating at 65 °C at 4 weeks) were sacrificed 4 weeks after stent placement. The rats in group C (AuNP-coated SEMS with local heating at 65 °C at 4 weeks) and group D (AuNP-coated SEMS with local heating at 65 °C at 4 and 8 weeks) were sacrificed 8 weeks after stent placement. The effectiveness of local heating was assessed by histopathology. All procedures were successful in all of the animals. Seven rats were excluded because of stent migration (n = 2) and death (n = 5). Granulation tissue formation-related variables were significantly higher in group A than in groups B-D (all p < 0.05). Heat-shock protein 70 (HSP70) and TUNEL expression were significantly lower in group A than in groups B-D (all p < 0.05). Granulation tissue formation-related variables were significantly higher in group C than in groups B and D (all p < 0.05). PTT using AuNP-coated SEMS successfully treated granulation tissue formation after stent placement in the rat esophagus.
采用支架技术的微创疗法目前受到支架邻近部位形成的支架诱导性肉芽组织的限制。本研究旨在探讨载金纳米颗粒（AuNP）涂层支架的光热疗法（PTT）治疗大鼠食管支架诱导性肉芽组织形成的效果。所有实验均获得本机构动物研究委员会的批准。制作了载 AuNP 的自膨式金属支架（SEMS），以便在近红外激光照射下进行 PTT。40 只大鼠随机分为 4 组（每组 10 只）。A 组（未涂层 SEMS）和 B 组（4 周时局部加热至 65°C 的 AuNP 涂层 SEMS）在支架放置 4 周后处死。C 组（4 周时局部加热至 65°C 的 AuNP 涂层 SEMS）和 D 组（4 周和 8 周时局部加热至 65°C 的 AuNP 涂层 SEMS）在支架放置 8 周后处死。通过组织病理学评估局部加热的效果。所有动物的所有程序均成功完成。7 只大鼠因支架移位（n=2）和死亡（n=5）而被排除。与肉芽组织形成相关的变量在 A 组显著高于 B-D 组（均 p<0.05）。与 A 组相比，B-D 组的热休克蛋白 70（HSP70）和 TUNEL 表达显著降低（均 p<0.05）。与 B 组和 D 组相比，C 组的与肉芽组织形成相关的变量显著升高（均 p<0.05）。载 AuNP 涂层 SEMS 的 PTT 成功治疗了大鼠食管支架放置后形成的肉芽组织。
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